Sequences from confirmation amplicon


Confirmation sequences for line 353G10 and hit At3g45440 are shown.
Line ID 353G10
Locus At3g45440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16665503
Primers used for PCR KF43 - 3144, show primer details
   
Sequence Name 08-K106971-0022-353-G10C-3144
GenBank Accession LN489752 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K106971-0022-353-G10C-3144
CGCAGAGCCTTCCTCTATATAAGGAAGTTCATTTCATTTGCAGTGGACAGGGTACCCGGG
GATCAGATTGTCGTTTCCCGCCTTCGGTTTTTCATTGGTAATGCTCCTGTATGCATGTAA
TTGAGAGCTGTAGCTATATCTTTAATGATTAAAAGTCTTCGACGCCATGAAAATGGCGGT
TTTTCGTCATTGAATAAGTATTGGTCAAGGCTACCATTGGGCATGTATTCAGATACCAGA
AGTAACTCACCTTTTCTTCTACAATACCCAAGAAGTGGAACCATATTCTTGTGCTTCAGA
TTACCCATGCTCACGATTTCAGCCACAAACTGCTTCATCCCTTCCTCTGCATCATGGGAC
ACTCTTTTCACTGCTATTTGTCCTTTGGAAGGCAGAGTTCCTTTGTACACTTTTCCAAAA
CCTCCCTTTCCAAGAAGTCCATCCTTGTTAAACCCATTCGTTGCAATGTACAGATCTTTA
TAAGAAAAACGGTGTGGGCCATATTCTTTTTCCCATTCTTCTCTTATTTCTGCATATTTC
CTTCTTCTGTAGACATAAGCTGCTCCAAGAGCCAAAAACACTATGATTGCCAGTAAAATG
AGTAGAACAATAAGGACCACTGATGGTCCCTTATTTTTAGCTCTATGACGAGGAACTCGA
GGAAGTTTAGTGACATCAAGGGTCTGCAATGACACCTTGT
   
Sequence Name 24-K106972-0022-353-G10C-KF43
GenBank Accession LN497011 [GenBank]
Sequencing primer gene specific primer, KF43: 5'-TTAAGCAGAAACAAGGTGTCATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K106972-0022-353-G10C-KF43
ATAAGGGACCATCAGTGGTCCTTATTGTTCAACTCATTTTACTGGCAATCATAGTGTTTT
AGGCTCTTGGAGCAGCTTATGTCTACAGAAGAAGGAAATATGCAGAAATAAGAGAAGAAT
GGGAAAAAGAATATGGCCCACACCGTTTTTCTTATAAAGATCTGTACATTGCAACGAATG
GGTTTAACAAGGATGGACTTCTTGGAAAGGGAGGTTTTGGAAAAGTGTACAAAGGAACTC
TGCCTTCCAAAGGACAAATAGCAGTGAAAAGAGTGTCCCATGATGCAGAGGAAGGGATGA
AGCAGTTTGTGGCTGAAATCGTGAGCATGGGTAATCTGAAGCACAAGAATATGGTTCCAC
TTCTTGGGTATTGTAGAAGAAAAGGTGAGTTACTTCTGGTATCTGAATACATGCCCAATG
GTAGCCTTGACCAATACTTATTCAATGACGAAAAACCGCCATTTTCATGGCGTCGAAGAC
TTTTAATCATTAAAGATATAGCTACAGCTCTCAATTACATGCATACAGGAGCATTACCAA
TGAAAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATG
AAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATC
CCTTACGTCAGTGGAGATA
Primers used for PCR 93wr - 8474, show primer details
   
Sequence Name 82-K118656-0022-353-G10C-93wr
GenBank Accession LN514243 [GenBank]
Sequencing primer gene specific primer, 93wr: 5'-AGCAAAGTTGTCAAAGTTTACCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K118656-0022-353-G10C-93wr
ATGTGGATCGTTGGCTGAGGAAGAAGTGTAAAAGGTCTTGGTACTGGGGGAGACAGGGGG
GTTTGATCCGATAATCAGCGGGGTAAAGGACCCGATACCAGGAGAATTTGGAGATATATC
AGGCAGCTCTAAGCTTCCGTTTAAGTATTGAACAATATCTTCCATAGAAGGTCTCAGGTC
TGGCACACCATTTGTGCAAAGCAACCCTAGTTTCAGTACCATTTCAACTTCCTCAGCTGA
GATTTCACCTCTCATTCTTGGATCTCTTGCTCCTAGCAAAGAAGCCATTTTCCAACATTC
GCAGACCCACTTAACTATATACCATCTTTCAGCTGACAACCCAGGTTCCACAGGTCTCCT
CCCACATGTTACTTCAAGTAAAAAGGCACCAAAGCCATACACATCCGTGGCAGTGCAAGC
TCCCACTGTAGCAAGCTCTGGTGCCATGTAGCCAATGGTTCCCACAGCAGCAGTTGTAGC
AGGGTCTTTCCCATGATCATGAAACCTTGCCATACCAAAGTCCCCTAACCTTCCATTAAA
TTCGGTGTCTAACATAACGTTAGAGGCCTTGATATCCCGGTGGAGAACAATTGTAGATGA
TTAAATATGGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCG
   
Sequence Name 56-K118655-0022-353-G10C-8409
GenBank Accession LN509246 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K118655-0022-353-G10C-8409
TTGAAGCCTATTTATCATCTACAATTGTTCTCCACCGGGATATCAAGGCCTCTAACGTTA
TGTTAGACACCGAATTTAATGGAAGGTTAGGGGACTTTGGTATGGCAAGGTTTCATGATC
ATGGGAAAGACCCTGCTACAACTGCTGCTGTGGGAACCATTGGCTACATGGCACCAGAGC
TTGCTACAGTGGGAGCTTGCACTGCCACGGATGTGTATGGCTTTGGTGCCTTTTTACTTG
AAGTAACATGTGGGAGGAGACCTGTGGAACCTGGGTTGTCAGCTGAAAGATGGTATATAG
TTAAGTGGGTCTGCGAATGTTGGAAAATGGCTTCTTTGCTAGGAGCAAGAGATCCAAGAA
TGAGAGGTGAAATCTCAGCTGAGGAAGTTGAAATGGTACTGAAACTAGGGTTGCTTTGCA
CAAATGGTGTGCCAGACCTGAGACCTTCTATGGAAGATATTGTTCAATACTTAAACGGAA
GCTTAGAGCTGCCTGATATATCTCCAAATTCTCCTGGTATCGGGTCCTTTACCCCGCTGA
TTATCGGATCAAACCCCCCTGTCTCCCCCAGTACCAAGACCTTTTACACTTCTTCCTCAG
CCAACGATTCCACATTTGTCACTCACTCAATTATCCATGGGGATGGACGGTAAACTTTGA
AAACTTTG

Last Updated on 06.12.2018 16:11