Sequences from confirmation amplicon


Confirmation sequences for line 354A04 and hit At3g14160 are shown.
Line ID 354A04
Locus At3g14160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4694966
Primers used for PCR 35ns - 8474, show primer details
   
Sequence Name 52-K114626-0022-354-A04C-35ns
GenBank Accession LN507897 [GenBank]
Sequencing primer gene specific primer, 35ns: 5'-AACTCGACCATTTGATGGTTCTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K114626-0022-354-A04C-35ns
AGAAGCAGTTAAAGAATCACAGTCCCTAGCGGCCTCAAATTCAAAGCAGACAAAGGGAGG
AGATGAAATACCATTTATGTTACCTGACATCTGTATTGTTAACTTCTACTCATCCACCGG
GAGACTTGGTCTCCATCAGGTCAGTATTTCAAACAACTTCTTGATATCATGAAGCTCAAA
GGTTGTATCATGAGTTTCAAATCCTTGATGTTTCAGGACAAAGATGAGAGCGAAAATTCC
ATCCGGAAGGGATTACCTGTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCA
   
Sequence Name 52-K114625-0022-354-A04C-8409
GenBank Accession LN507896 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K114625-0022-354-A04C-8409
TTTACACAGGTAATCCCTTCCGGATGGAATTTTCGCTCTCATCTTTGTCCTGAAACATCA
AGGATTTGAAACTCATGATACAACCTTTGAGCTTCATGATATCAAGAAGTTGTTTGAAAT
ACTGACCTGATGGAGACCAAGTCTCCCGGTGGATGAGTAGAAGTTAACAATACAGATGTC
AGGTAACATAAATGGTATTTCATCTCCTCCCTTTGTCTGCTTTGAATTTGAGGCCGCTAG
GGACTGTGATTCTTTAACTGCTTTCTCAACAAACTGATTGAATTCAGCAGGAATTCTTGG
CGCAGTAGAACCATCAATGGGTCGAGT

Last Updated on 06.12.2018 16:11