Sequences from confirmation amplicon


Confirmation sequences for line 354B10 and hit At5g53100 are shown.
Line ID 354B10
Locus At5g53100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21526377
Primers used for PCR ZZ06 - 8474, show primer details
   
Sequence Name 41-K020244-022-354-B10C-8409
GenBank Accession LN503915 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K020244-022-354-B10C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTAAAGGAGGAAGATGAAATGGAATTTGTAA
GTGAGAAGCCATTATTCTCTGCAAAAGAATCTCTTGCACAACATTAAACCATCCTCTCAT
CCACTGAATCCACCCTAAACCATCATTCTTCTTCATCTCACTACTCATCTTCTTCTTCTC
TCTAGAGTTCACAAATCACAACACATCTATAGAGAGAATTAAAGACAAAAGCCTGTTATA
TAAAGATAATTATTACGTCGAAATCAAGGAACATGATAAACATTTTTGGGTTAGTTAAAG
CCTGTGACGCCTCGGACTTGTTGGTATACGTACTTCGTCTGCCGTTACAACTTACAACAA
TATAATTAGTTGGTCATTAATTGACCATTAACTAATTACTAGTACTAATTATTGTTATTA
CTATTACTACATGAGAACCCGCCTTTTTCGTA
   
Sequence Name 42-K020244-022-354-B10C-ZZ06
GenBank Accession LN504304 [GenBank]
Sequencing primer gene specific primer, ZZ06: 5'-ACACATATTATACGAAAAAGGCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K020244-022-354-B10C-ZZ06
TCATGTAGTAATAGTAATAACAATAATTAGTACTAGTAATTAGTTAATGGTCAATTAATG
ACCAACTAATTATATTGTTGTAAGTTGTAACGGCAGACGAAGTACGTATACCAACAAGTC
CGAGGCGTCACAGGCTTTAACTAACCCAAAAATGTTTATCATGTTCCTTGATTTCGACGT
AATAATTATCTTTATATAACAGGCTTTTGTCTTTAATTCTCTCTATAGATGTGTTGTGAT
TTGTGAACTCTAGAGAGAAGAAGAAGATGAGTAGTGAGATGAAGAAGAATGATGGTTTAG
GGTGGATTCAGTGGATGAGAGGATGGTTTAATGTTGTGCAAGAGATTCTTTTGCAGAGAA
TAATGGCTTCTCACTTACAAATTCCATTTCATCTTCCTCCTTTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGC

Last Updated on 06.12.2018 16:11