Sequences from confirmation amplicon


Confirmation sequences for line 354F03 and hit At3g13380 are shown.
Line ID 354F03
Locus At3g13380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4347620
Primers used for PCR EQ33 - 8474, show primer details
   
Sequence Name 07-K103004-0022-354-F03C-8409
GenBank Accession LN489226 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K103004-0022-354-F03C-8409
GCCATTTACATTGAATCTCCGGAGGAGAAGTTGTTTCCTTGCAATAAAGACTCCGGAGAT
TAGACAACGCCGTGAGATTATTGAGATTTAGGGTTCCGGTAAGACCACCGTTTCGTAGAT
CAAGTCCAATGACTCTACCATCGCTAGAACAAGAGACGCCTCGCCAAGTACATGGGTCAC
GACCCGACCCGTATCTCCAGTTACCTAAAAAGTTGGTAGGATCCGACTTGATAGAGGTTT
GCTTAAACGCCGTTAGCAGAGCTGTGTCGTTAACGTCGTCGCTGAGTAGCCGTCTGCCAC
GAGAGTCCACCGTTAGAAACAAGACGAGCAAACAG
   
Sequence Name 55-K103005-0022-354-F03C-EQ33
GenBank Accession LN508776 [GenBank]
Sequencing primer gene specific primer, EQ33: 5'-TCTGTTTGCTCGTCTTGTTTCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K103005-0022-354-F03C-EQ33
TCAGCGAGACGTTACGACACAGCTCTGCTAACGGCGTTTAAGCAAACCTCTATCAAGTCG
GATCCTACCAACTTTTTAGGTAACTGGAGATACGGGTCGGGTCGTGACCCATGTACTTGG
CGAGGCGTCTCTTGTTCTAGCGATGGTAGAGTCATTGGACTTGATCTACGAAACGGTGGT
CTTACCGGAACCCTAAATCTCAATAATCTCACGGCGTTGTCTAATCTCCGGAGTCTTTAT
TTGCAAGGAAACAACTTCTCCTCCGGAGATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATNGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACNTTCACTCCCNAAATGG
Primers used for PCR KF44 - 8474, show primer details
   
Sequence Name 83-K118656-0022-354-F03C-KF44
GenBank Accession LN514359 [GenBank]
Sequencing primer gene specific primer, KF44: 5'-TTAACGTTTCGAGGAGCTTACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K118656-0022-354-F03C-KF44
CTGTTCTGTGATAAGCTGAATACGGTGAGGTTCTCACAGAGACCAAAGCTGAGACGAGAG
AAGTCGCCGGTGACATTGTTGCCGGATAAGTCGAGGTGTTTCAGAGAATTCGGAAAATCG
GCGATGAAAGTCTCCGGAATCTCATCGGAGAAGCGATTGTTCGAGAGATCAACGGTTGTG
ATTCTCTTGTTACTCGCCGAAGGAGAAGACTTCAATTTCCCGGCGAGCTTGTTGTGCGAG
AAATTGACAGAAACCAGATTTAAACAGGTGGAGAAAACGTAATCCACTATTGAAGAGTCC
GTAAGCGAGTTTGAAGATAAATCCAGAACCAATGAGTATGATGGTTTTCAAACACTGATA
GTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATG
AAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATC
CCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTC
TTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGA
GGCATCTTCAACGATGGCCTTTCCTTTATCGCAATGATGGCATTTGTAGGAGCCACCTTC
CTTTTCCACTATCTTCACAATAAAGTGACAGATAGCTGGGCAATGGAATCCGAGGAGGTT
TCCGGATATTACCCTTTGTTGAAAAGTCTCAATTGCCCTTTGGTCTTCTGAGACTGTATC
TNTGATATTTTTGGAGTAGACAAGCGTGTCGTGCTCCACCATGTTGACGAAGATTTTCTT
CTTGTCATTGAGTCGTAAGAGACTCTGTATGAACTGTTCGCCAGTCTTTACGGCGAGTTC
TGTTAGGTCCTCTATTTGAATCTTTGACTCCCTGGGAATTGGGGATCCTCTAGAGTCGAC
ATGCATGCATTGGTT
   
Sequence Name 57-K118655-0022-354-F03C-8409
GenBank Accession LN509553 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K118655-0022-354-F03C-8409
ATCCCAATTCCCATGGAGTCAAGATTCAAATAGAGGACCTAACAGAACTCGCCGTAAAGA
CTGGCGAACAGTTCATACAGAGTCTCTTACGACTCAATGACAAGAAGAAAATCTTCGTCA
ACATGGTGGAGCACGACACGCTTGTCTACTCCAAAAATATCAAAGATACAGTCTCAGAAG
ACCAAAGGGCAATTGAGACTTTTCAACAAAGGGTAATATCCGGAAACCTCCTCGGATTCC
ATTGCCCAGCTATCTGTCACTTTATTGTGAAGATAGTGGAAAAGGAAGGTGGCTCCTACA
AATGCCATCATTGCGATAAAGGAAAGGCCATCGTTGAAGATGCCTCTGCCGACAGTGGTC
CCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGT
CTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCC
ACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAG
GGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAAAA
CCATCATACTCATTGGTTCTGGATTTATCTTCAAACTCGCTTACGGACTCTTCAATAGTG
GATTACGTTTTCTCCACCTGTTTAAATCTGGTTTCTGTCAATTTCTCGCACAACAAGCTC
GCCGGGAAATTGAAGTCTTCTCCTTCGGCGAGTAACAAGAGAATCACAACCGTTGATCTC
TCGAACAATCGCTTCTCCGATGAGATTCCGGAGACTTTCATCGCCGATNTTCCGAATTCT
CTGAAACACCTCGACTTATCCGGCAACAATGTCACCGGCGACTTCTCTCGTCTCAGCTTT
GGTCTCTGTGAGAACCTCACCGTATTCAGCTTATCA
Primers used for PCR KF44 - 3144, show primer details
   
Sequence Name 46-K119798-0022-354-F03C-KF44
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, KF44: 5'-TTAACGTTTCGAGGAGCTTACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K119798-0022-354-F03C-KF44
TGTTCTGTGATAAGCTGAATACGGTGAGGTTCTCCAGAGACCAAAGCTGAGACGAGAGAA
GTCGCCGGTGACATTGTTGCCGGATAAGTCGAGGTGTTTCAGAGAATTCGGAAAATCGGC
GATGAAAGTCTCCGGAATCTCATCGGAGAAGCGATTGTTCGAGAGATCAACGGTTGTGAT
TCTCTTGTTACTCGCCGAAGGAGAAGACTTCAATTTCCCGGCGAGCTTGTTGTGCGAGAA
ATTGACAGAAACCAGATTTAAACAGGTGGAGAAAACGTAATCCACTATTGAAGAGTCCGT
AAGCGAGTTTGAAGATAAATCCAGAACCAATGAGTATGATGGTTTTCAAACACTGATAGT
TTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAA
ATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCC
TTACGTCAGTGGAGATATCAAACCAATCCA
   
Sequence Name 10-K119797-0022-354-F03C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K119797-0022-354-F03C-3144
CAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGAT
CAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAAAACCATCATACTCAT
TGGTTCTGGATTTATCTTCAAACTCGCTTACGGACTCTTCAATAGTGGATTACGTTTTCT
CCACCTGTTTAAATCTGGTTTCTGTCAATTTCTCGCACAACAAGCTCGCCGGGAAATTGA
AGTCTTCTCCTTCGGCGAGTAACAAGAGAATCACAACCGTTGATCTCTCGAACAATCGCT
TCTCCGATGAGATTCCGGAGACTTTCATCGCCGATTTTCCGAATTCTCTGAAACACCTCG
ACTTATCCGGCAACAATGTCACCGGCGACTTCTCTCGTCTCAGCTTTGGTCTCTGTGAGA
ACCTCACCGTATTCAGCTTATCACAGAACAGCATCTCCGGCGACAGATTTCCGGTTAGTC
TCTCAAATAGTAAGCTCCTCAAAACGTTAA

Last Updated on 06.12.2018 16:11