Sequences from confirmation amplicon


Confirmation sequences for line 355A10 and hit At4g26090 are shown.
Line ID 355A10
Locus At4g26090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13226345
Primers used for PCR BV71 - 8474, show primer details
   
Sequence Name 41-K106149-0022-355-A10C-BV71
GenBank Accession LN504086 [GenBank]
Sequencing primer gene specific primer, BV71: 5'-GATATTTTCTCATTGGGGATCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K106149-0022-355-A10C-BV71
GACGGAGATAGAAACCAGGTGAAGATGCATAATGTGGTCAGAAGCTTTGCATTGTGGATG
GCATCTGAACAGGGGACTTATAAGGAGCTGATCCTAGTTGAGCCTAGCATGGGACATACT
GAAGCTCCTAAAGCAGAAAACTGGCGACAAGCGTTGGTGATCTCATTGTTAGATAACAGA
ATCCAGACCTTGCCTGAAAAACTCATATGCCCGAAACTGACAACACTGATGCTCCAACAG
AACAGCTCTTTGAAGAAGATTCCAACAGGGTTTTTCATGCATATGCCTGTTCTCAGAGTC
TTGGACTTGTCGTTCACAAGTATCACTGAGATTCCGTTGTCTATCAAGTATTTGGTGGAG
TTGTTTCACGGTTATTTGGCGTTTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 57-K106150-0022-355-A10C-8409
GenBank Accession LN509423 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K106150-0022-355-A10C-8409
TCGGAATGAGCCATTTACAATTGAATTATCCTGAAACGCCAATAACCGTGAAACAACTCC
ACCAAATACTTGATAGACAACGGAATCTCAGTGATACTTGTGAACGACAAGTCCAAGACT
CTGAGAACAGGCATATGCATGAAAAACCCTGTTGGAATCTTCTTCAAAGAGCTGTTCTGT
TGGAGCATCAGTGTTGTCAGTTTCGGGCATATGAGTTTTTCAGGCAAGGTCTGGATTCTG
TTATCTAACAATGAGATCACCAACGCTTGTCGCCAGTTTTCTGCTTTAGGAGCTTCAGTA
TGTCCCATGCTAGGCTCAACTAGGATCAGCTCCTTATAAGTCCCCTGTTCAGATGCCATC
CACAATGCAAAGCTTCTGACCACATTATGCATCTTCACCTGTGTTTTCTCATCTCCGGTT
TCCAACAAACATGCCGCTTTCAGATCCCCAATGAGAAAATATCAGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT

Last Updated on 06.12.2018 16:11