Sequences from confirmation amplicon


Confirmation sequences for line 355C01 and hit At1g32640 are shown.
Line ID 355C01
Locus At1g32640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11799159
Primers used for PCR MM36 - 8474, show primer details
   
Sequence Name 02-K019980-022-355-C01C-MM36
GenBank Accession LN486842 [GenBank]
Sequencing primer gene specific primer, MM36: 5'-GTTCTTATTCGGGTCAAACACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K019980-022-355-C01C-MM36
AAAAGAAAGAGGTCGATGGTTTTGAACGAAGATAAAGTTCTATCATTCGGAGATAAAACC
GCCGGAGAATCAGATCACTCCGATCTAGAAGCTTCCGTCGTGAAAGAAGTAGCAGTAGAG
AAACGTCCAAAGAAACGAGGAAGAAAGCCAGCAAACGGTAGAGAAGAGCCACTAAACCAC
GTCGAAGCAGAGAGACAAAGACGCGAGAAACTAAACCAAAGATTCTACGCGTTACGAGCG
GTTGTACCAAACGTTTCAAAAATGGATAAAGCTTCGTTACTCGGTGACGCAATCGCTTAC
ATCAACGAGCTTAAATCCAAAGTAGTCAAAACAGAGTCAGAGAAACTCCAAATCAAGAAC
CAGCTCGAGGAAGTGAAACTCGAGCTCGCCGGAAGAAAAGCGAGTGCTAGTGGAGGAGAT
ATGTCGTCTTCGTGTTCTTCGATTAAACCGGTGGGGATGGAGATTGAAGTGAAGATAATT
GGTTGGGACGCAATGATTAGAGTTGAATCTAGTAAGAGGAATCATCCGGCGGCGAGGTTG
ATGTCGGCGTTGATGGATTTGGAGTTGGAAGTGAATATATCCTGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGGAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGGCGAAAGCAATAAA
   
Sequence Name 06-K019980-022-355-C01C-8409
GenBank Accession LN488609 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K019980-022-355-C01C-8409
CCAGGATATATTCACTTCCAACTCCAAATCCATCAACGCCGACATCAACCTCGCCGCCGG
ATGATTCCTCTTACTAGATTCAACTCTAATCATTGCGTCCCAACCAATTATCTTCACTTC
AATCTCCATCCCCACCGGTTTAATCGAAGAACACGAAGACGACATATCTCCTCCACTAGC
ACTCGCTTTTCTTCCGGCGAGCTCGAGTTTCACTTCCTCGAGCTGGTTCTTGATTTGGAG
TTTCTCTGACTCTGTTTTGACTACTTTGGATTTAAGCTCGTTGATGTAAGCGATTGCGTC
ACCGAGTAACGAAGCTTTATCCATTTTTGAAACGTTTGGTACAACCGCTCGTAACGCGTA
GAATCTTTGGTTTAGTTTCTCGCGTCTTTGTCTCTCTGCTTCGACGTGGTTTAGTGGCTC
TTCTCTACCGTTTGCTGGCTTTCTTCCTCGTTTCTTTGGACGTTTCTCTACTGCTACTTC
TTTCACGACGGAAGCTTCTAGATCGGAGTGATCTGATTCTCCGGCGGTTTTATCTCCGAA
TGATAGAACTTTATCTTCGTTCAAAACCATCGACCTCTTTCTTTTGTTTTCGAATTGTGT
TTGACCC

Last Updated on 06.12.2018 16:11