Sequences from confirmation amplicon


Confirmation sequences for line 355H05 and hit At3g61120 are shown.
Line ID 355H05
Locus At3g61120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22619341
Primers used for PCR CP09 - 8474, show primer details
   
Sequence Name 12-K031007-022-355-H05C-8409
GenBank Accession LN491450 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K031007-022-355-H05C-8409
GCCATTTACATTGAATATGGCTCATGTGTGCTATAATGCAAATACTCCTCGTAATCCATT
ACTTCCTATAATTGTACATTTCCTAATTTCTGCTACTAATAATCGTTTTGAATCGTTGAA
AAAGGAATTTGGTTGGGGAAGATCTTGAAGGGATGAGTATAAAGGAATTGCAGACGCTGG
AGAGACAGCTTGAAGGAGCTCTTTCCGCAACCCGAAAACAAAAGGCATGACATATTTCAT
ACGTAGACCAATTTTTCTTGCGCATCATTTAGACCTGCAAAATACATTATTGAAATGTAT
ATCTTTATATGCATGTATGTATATAGACGCAGGTTATGATGGAACAAATGGAA
   
Sequence Name 13-K031007-022-355-H05C-CP09
GenBank Accession LN491914 [GenBank]
Sequencing primer gene specific primer, CP09: 5'-CTTCCATTTGTTCCATCATAACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K031007-022-355-H05C-CP09
AAGATATACTTTCATAATGTATTTTGCAGGTCTAAATGATGCGCAAGAAAAATTGGTCTA
CGTATGAAATATGTCATGCCTTTTGTTTTCGGGTTGCGGAAAGAGCTCCTTCAAGCTGTC
TCTCCAGCGTCTGCAATTCCTTTATACTCATCCCTTCAAGATCTTCCCCAACCAAATTCC
TTTTTCAACGATTCAAAACGATTATTAGTAGCAGAAATTAGGAAATGTACAATTATAGGA
AGTAATGGATTACGAGGAGTATTTGCATTATAGCACACATGAGCCATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC
TCCCAAATG

Last Updated on 06.12.2018 16:11