Sequences from confirmation amplicon


Confirmation sequences for line 356C12 and hit At1g21720 are shown.
Line ID 356C12
Locus At1g21720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7628056
Primers used for PCR 15ta - 8474, show primer details
   
Sequence Name 32-K116770-0022-356-C12C-15ta
GenBank Accession LN500732 [GenBank]
Sequencing primer gene specific primer, 15ta: 5'-AGCTTGTTAGCCATACTCCATTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K116770-0022-356-C12C-15ta
CACATGAGGATTTTCAGGAAGCTGAGGAATTGTTCGAGACAATATCGCAAGCACTTCTCT
CATCTGTTGACCGTGATTGTCTGAGTGGTTGGGGAGGGCATGTTTACATTGTGTAAGTTC
TCATTTCTTCTCTTCTTTTCCACACATTTGTTATCCAAAATTAGTTCTTAAAACAAACTT
CCATGGTGATGTGTTGTACAGAACACCAACAGAGATTAAGGAGAGGATCCTAAAGGAGAT
GATCAACTTGAATAGAAGAAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 08-K116769-0022-356-C12C-8409
GenBank Accession LN489898 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K116769-0022-356-C12C-8409
ATTTCATTGAATATATCCTGCTTCTTCTATTCAGTTGATCATCTCCTTTAGGATCCTCTC
CTTAATCTCTGTTGGTGTTCTGTACAACACATCACCATGGAAGTTTGTTTTAAGAACTAA
TTTTGGATAACAAATGTGTGGAAAAGAAGAGAAGAAATGAGAACTTACACAATGTAAACA
TGCCCTCCCCAACCACTCAGACAATCACGGTCAACAGATGAGAGAAGTGCTTGCGATATT
GTCTCGAACAATTCCTCAGCTTCCTGAAAATCCTCATGTGTTAAGCCAGAGTCTCGATAC
ATAAGTCTAGAGTAGTACAATGGAGTATGGCTAACAAGCT

Last Updated on 06.12.2018 16:11