Sequences from confirmation amplicon


Confirmation sequences for line 357G12 and hit At1g07480 are shown.
Line ID 357G12
Locus At1g07480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2297689
Primers used for PCR BV74 - 8474, show primer details
   
Sequence Name 42-K106149-0022-357-G12C-BV74
GenBank Accession LN504471 [GenBank]
Sequencing primer gene specific primer, BV74: 5'-TATCGTTTTGTACAGCAACAGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K106149-0022-357-G12C-BV74
AAAAAGAGGGATTTAGCAGATAATAATCATTCACTTACTCTAAAACAATATCCAACATTT
TTGTTTGGCTTTCCTTACCGTTTGGAGCAGGAGTTCTGGCCTCGTTAAACTCTTCACTGG
GTCCTTGATAACTATATATCTAAAACGCAGTAAATGAAATTCATTGAATCTAGCAAACGT
ACTTGGGTGAAAGGGTAATAGATAGTATCTCTAGACAAAAGTTACATGTATGATGGCAAA
TCCAAATGCAATATCTTTGATGGGTAGTGCGGAGGGATCCAGTTGTACTAAGGCAGAAAA
GTTTCTAGCTACTGATTTGTGAGCATGTCGAAGAAGTATATATCTACTGAAACTTTTGAC
TAAATGAAAAGCAGCATTCTTACATTTGGTGTAGAGAGCATTTCATCATAAGGGTCAGGC
ATTGGCCCATCAACCTGAGGAATCTTCGATGATGAGCTGAAGAAGTCTCGTGGTACCTTT
CTATCGCCAATAGAGGTTATGCGGAATGCATCACACTCAAAGTTTGCCTGCAAAATTGTG
CTCAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATT
   
Sequence Name 58-K106150-0022-357-G12C-8409
GenBank Accession LN509711 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K106150-0022-357-G12C-8409
GAGCCTTTACATTGAATATTGAGCACAATTTTGCAGGCAAACTTTGAGTGTGATGCATTC
CGCATAACCTCTATTGGCGATAGAAAGGTACCACGAGACTTCTTCAGCTCATCATCGAAG
ATTCCTCAGGTTGATGGGCCAATGCCTGACCCTTATGATGAAATGCTCTCTACACCAAAT
GTAAGAATGCTGCTTTTCATTTAGTCAAAAGTTTCAGTAGATATATACTTCTTCGACATG
CTCACAAATCAGTAGCTAGAAACTTTTCTGCCTTAGTACAACTGGATCCCTCCGCACTAC
CCATCAAAGATATTGCATTTGGATTTGCCATCATACATGTAACTTTTGTCTAGAGATACT
ATCTATTACCCTTTCACCCAAGTACGTTTGCTAGATTCAATGAATTTCATTTACTGCGTT
TTAGATATATAGTTATCAAGGACCCAGTGAAGAGTTTAACGAGGCCAGAACTCCTGCTCC
AAACGGTAAGGAAAGCCAAACAAAAATGTTGGATATTGTTTTAGAGTAAGTGAATGATTA
TTATCTGCTAAATTCCCTCTTTTTTTTGTTAGAGATCCAAACGAGCACTCCTGTTGCTGT
ACAAAACGATA

Last Updated on 06.12.2018 16:11