Sequences from confirmation amplicon


Confirmation sequences for line 358B01 and hit At5g09350 are shown.
Line ID 358B01
Locus At5g09350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2901328
Primers used for PCR BF67 - 8474, show primer details
   
Sequence Name 29-K030141-022-358-B01C-8409
GenBank Accession LN499163 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K030141-022-358-B01C-8409
ATTGAATATGATGGTCATGTAGATTTTTGAGGAAAAGAACTATGAAATTAACAGCCATAT
GGTAGCTAAACAAAATATCACCTACCAAAAAAATGAGATATAAGTTGAACCGCAAGATGT
TCCTGCCGACAGTCATCACCGCTCTTCACAATGATCTGCAAAAGCCAGAAAAGAATTGGA
ATAATAGGTCAAACAACAAAATTAACAGTTTCTTAGGGTTGTTTTAATATAGTGTACAGA
AATTCCTAGACAACAACTTTCAATGTCAACAAGTATAAACCAACTTTTACATTCAGCAGG
AAAGAAATTAAAAACAGAAGCTTATCAAAAGCAGATCCCCACAATGAGTTTCTAAAGACT
CAAAGCATATAGCCATATACAAAGGAATGAATGTAAAAACACTTCATGTATTTTCACTGT
TGAAAATTTTTGAACTTTAAGTAGAGATACGCAACCATTTATCTGACAAGAAGTACCATT
ATGTATTTTATTTATTGCTACACAAGATGTTCAAGTGGAGAAATATACTCTACAAGGAAA
AGCCAGCGGAAGATAAGGAGGTTTGCTTTATCCAG
   
Sequence Name 29-K030141-022-358-B01C-BF67
GenBank Accession LN499164 [GenBank]
Sequencing primer gene specific primer, BF67: 5'-TTGCGCTCTGTAAGTACTGGATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K030141-022-358-B01C-BF67
TCGCTGGCTTTTCCTTNTAGAGTATATTTCTCCACTTGAACATCTTGTGTAGCAATAAAT
AAAATACATAATGGTACTTCTTGTCAGATAAATGGTTGCGTATCTCTACTTAAAGTTCAA
AAATTTTCAACAGTGAAAATACATGAAGTGTTTTTACATTCATTCCTTTGTATATGGCTA
TATGCTTTGAGTCTTTAGAAACTCATTGTGGGGATCTGCTTTTGATAAGCTTCTGTTTTT
AATTTCTTTCCTGCTGAATGTAAAAGTTGGTTTATACTTGTTGACATTGAAAGTTGTTGT
CTAGGAATTTCTGTACACTATATTAAAACAACCCTAAGAAACTGTTAATTTTGTTGTTTG
ACCTATTATTCCAATTCTTTTCTGGCTTTTGCAGATCATTGTGAAGAGCGGTGATGACTG
TCGGCAGGAACATCTTGCGGTTCAACTTATATCTCATTTTTTTGGTAGGTGATATTTTGT
TTAGCTACCATATGGCTGTTAATTTCATAGTTCTTTTCCTCAAAAATCTACATGACCATC
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT

Last Updated on 06.12.2018 16:11