Sequences from confirmation amplicon


Confirmation sequences for line 358F06 and hit At1g75840 are shown.
Line ID 358F06
Locus At1g75840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28476278
Primers used for PCR MB12 - 8474, show primer details
   
Sequence Name 31-K025267-022-358-F06C-8409
GenBank Accession LN500003 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K025267-022-358-F06C-8409
CGGACATGAAGCCATTTACATGAAGCGGCGCTCTCTACGCCCCGCCGCTTCATATCCATT
GCTGATCCATATTTGTCATTTTGGACTTCATGTTCAATTTGTATGTATTAGTCAAATTTG
CAGATATGAGGATCTATAATCTTTTCCATTATTGTGTGTGTTAATGATGATCCACTATTG
TGTGTGTTTTAGTTTAACAAACTCTATGACTTGGTTACTGAATATCAGGACTATGTTCCA
ACTGTTTTCGACAACTTCAGTGCTAATGTGGTTGTAGATGGGAACACGGTGAATCTTGGA
TTGTGGGATACAGCTGGTAAATAATCCTTTTGAAACTCATCAAATCTGGTTTTCTTGTTT
TTTTGTGGTTTCATTTTGACTGATAATTCTTGGGTTTGTGCAGGTCAAGAAGACTATAAC
AGGTTAAGACCGTTGAGTTACCGTGGTGCCGATGTCTTCATTCTTGCATTCTCGCTTATT
AGCAAAGCTAGCTACGAGAATGTAGCC
   
Sequence Name 32-K025267-022-358-F06C-MB12
GenBank Accession LN500427 [GenBank]
Sequencing primer gene specific primer, MB12: 5'-TAACCTTCTTGGCTACATTCTCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K025267-022-358-F06C-MB12
TGCTAATAAGCGAGAATGCAAGAATGAAGACATCGGCACCACGGTAACTCAACGGTCTTA
ACCTGTTATAGTCTTCTTGACCTGCACAAACCCAAGAATTATCAGTCAAAATGAAACCAC
AAAAAAACAAGAAAACCAGATTTGATGAGTTTCAAAAGGATTATTTACCAGCTGTATCCC
ACAATCCAAGATTCACCGTGTTCCCATCTACAACCACATTAGCACTGAAGTTGTCGAAAA
CAGTTGGAACATAGTCCTGATATTCAGTAACCAAGTCATAGAGTTTGTTAAACTAAAACA
CACACAATAGTGGATCATCATTAACACACACAATAATGGAAAAGATTATAGATCCTCATA
TCTGCAAATTTGACTAATACATACAAATTGAACATGAAGTCCAAAATGACAAATATGGAT
CAGCAATGGATATGAAGCGGCGGGGCGTAGAGAGCGCCGCTTCATGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11