Sequences from confirmation amplicon


Confirmation sequences for line 358F09 and hit At4g02660 are shown.
Line ID 358F09
Locus At4g02660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1174239
Primers used for PCR HM35 - 8474, show primer details
   
Sequence Name 39-K032416-022-358-F09C-8409
GenBank Accession LN503253 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K032416-022-358-F09C-8409
ATGATGATGATGAAGAACACAGCACAAAAACCAAACGACGAAATTGATATTTATTTTTTT
ATTTCGTTAATCGATCAATGAAATAGTTTTCTTCAGCGTAAATCGATCTCTAAATCTCGA
TTTTCTGCGTTCTCCTTTCAAAATCTGTTTCAGTATTCTTCTCAGATTATCAAGGAGGAA
GGAGAAATGAACTTGTTGTTGGTGGTGTGAATCTCGCTAATCGCAATTTTGGATCTTAGA
TTTTGTTCTTGCTACCAAACCCTAATCTCACACATTGCACCAGCATACATAGTTTTGATG
TCTGGGTTGTTGTTCGTCTTTATTGATTCCGTTTCCTTAATCAAAAGAAACGGGTTGATT
TCTGAGCCCCGATTCGGATCCGGGTCGTGTCCGACCGATTCAGGAGTGGTTATCGGATGA
ATGAATCTGGAATCGAAACTGTGGAACAATGAAATGGGGAACATTGCTTAAGGACTTGAA
GGATAAGGTCGGAGTAGCTGAAACAACCGCCGATTTAATCGCCGGAGAAGCCATCAGTGA
CCCCACTACTCCACCGTCCTCTTCCCAAGCTTCTCCATCCTCTAGTTTCGCCGCCTTAGC
TCAACATGACTTCAATTTATTATCTCCTACAAGGTATTTTACTTTTATAATGTGCACTTT
GACTGATCAATGTTGGTATAAGTAAATCAATGATTCTCTCTTATTT
   
Sequence Name 39-K032416-022-358-F09C-HM35
GenBank Accession LN503254 [GenBank]
Sequencing primer gene specific primer, HM35: 5'-TCTCGGCTTTTCAAGGAGTAAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K032416-022-358-F09C-HM35
CATTGATCAGTCAAGTGCACATTATAAAAGTAAAATACCTTGTAGGAGATAATAAATTGA
AGTCATGTTGAGCTAAGGCGGCGAAACTAGAGGATGGAGAAGCTTGGGAAGAGGACGGTG
GAGTAGTGGGGTCACTGATGGCTTCTCCGGCGATTAAATCGGCGGTTGTTTCAGCTACTC
CGACCTTATCCTTCAAGTCCTTAAGCAATGTTCCCCATTTCATTGTTCCACAGTTTCGAT
TCCAGATTCATTCATCCGATAACCACTCCTGAATCGGTCGGACACGACCCGGATCCGAAT
CGGGGCTCAGAAATCAACCCGTTTCTTTTGATTAAGGAAACGGAATCAATAAAGACGAAC
AACAACCCAGACATCAAAACTATGTATGCTGGTGCAATGTGTGAGATTAGGGTTTGGTAG
CAAGAACAAAATCTAAGATCCAAAATTGCGATTAGCGAGATTCACACCACCAACAACAAG
TTCATTTCTCCTTCCTCCTTGATAATCTGAGAAGAATACTGAAACAGATTTTGAAAGGAG
AACGCAGAAAATCGAGATTTAGAGATCGATTTACGCTGAAGAAAACTATTTCATTGATCG
ATTAACGAAATAAAAAAATAAATATCAATTTCGTCGTTTGGTTTTTGTGCTGTGTTCTTC
ATCATCATCATCGTCTTCGTGTCTCCGCGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAACAAATTATTCTATTCGGAAATCTTA

Last Updated on 06.12.2018 16:11