Sequences from confirmation amplicon


Confirmation sequences for line 359C09 and hit At3g25600 are shown.
Line ID 359C09
Locus At3g25600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9307640
Primers used for PCR 24sa - 8474, show primer details
   
Sequence Name 29-K116272-0022-359-C09C-24sa
GenBank Accession LN499441 [GenBank]
Sequencing primer gene specific primer, 24sa: 5'-TCAATCCCACTTTAATTTGTCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K116272-0022-359-C09C-24sa
TAAATCCATAGATAATGGTAATAAAATTTTTGAATTTACTATTTTCAACTAAAAATATTA
TCAACTTCTTCTCCAAAAACGCGGTTTCTTCGAGTTTAAGAAACTATTCATCTCCAAACT
TTCAAAAAAAAACCTAAAACAAAAAAAATCTCTTTCCTTCTTCTTTCTCCATCAATGGCG
TCAACAAAACCAACCGATCAAATCAAACAACTCAAAGATATCTTCGCTCGTTTCGACATG
GACAAGGACGGAAGCTTAACGCAGCTAGAACTCGCCGCTCTTCTGCGTTCTCTCGGAATC
AAACCTCGCGGCGATCAAATCTCTCTTCTGTTGAACCAAATCGACCGTAACGGTAACGGA
TCCGTAGAGTTCGACGAGCTGGTCGTGGCGATATTGCCGGATATAAACGAAGAGGTTTTG
ATAAATCAAGAACAGTTGATGGAGGTTTTGATAAATCAAGAACAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 05-K116271-0022-359-C09C-8409
GenBank Accession LN488502 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K116271-0022-359-C09C-8409
AAACCTCCATCAACTGTTCTTGATTTATCAAAACCTCTTCGTTTATATCCGGCAATATCG
CCACGACCAGCTCGTCGAACTCTACGGATCCGTTACCGTTACGGTCGATTTGGTTCAACA
GAAGAGAGATTTGATCGCCGCGAGGTTTGATTCCGAGAGAACGCAGAAGAGCGGCGAGTT
CTAGCTGCGTTAAGCTTCCGTCCTTGTCCATGTCGAAACGAGCGAAGATATCTTTGAGTT
GTTTGATTTGATCGGTTGGTTTTGTTGACGCCATTGATGGAGAAAGAAGAAGGAAAGAGA
TTTTTTTTGTTTTAGGTTTTTTTTTGAAAGTTTGGAGATGAATAGTTTCTTAAACTCGAA
GAAACCGCGTTTTTGGAGAAGAAGTTGATAATATTTTTAGTTGAAATAGTAAATTCAAAA
TTTTATTACATTATCTATAGATTTATAGATTGGTACTGTTTCAATTATTGATAAGATATT
AATATGTGGAGACAATAAGGGGGG

Last Updated on 06.12.2018 16:11