Sequences from confirmation amplicon


Confirmation sequences for line 360C07 and hit At1g78700 are shown.
Line ID 360C07
Locus At1g78700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29600240
Primers used for PCR 22aq - 8474, show primer details
   
Sequence Name 15-K106755-0022-360-C07C-8409
GenBank Accession LN492990 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K106755-0022-360-C07C-8409
ATTGAATCATTAACAAAAACTGAATGATTTTGTATCCAAAATTCAAAACTTTCATGTACT
TGAAGCGTAGAGAACAAAACTAAGATCCGGATTCGAGAGACAAACCTTGCGGTAAGTAGT
TCCATCAGGTTCGACGATCCAACCAGCTTCGTTACAGAGTGCTTTAAGCACTTCGTTGTT
GTCGCAATGCTTCGGAAGCTCGTAATTACCGTACATTCTTAATCCGGTGAAGATCTTAGC
TGCGATTGCTCTCCGTCGTCTTTCTCTTCTCTTGTTGTTCTCTCTTTCCCTCCATGTCGG
CATTCTCGTCCCTGATGTCATCACTACTCTCTGTTTCTTCTTCCTCTGAAAACTCTCTGT
TTCTTCTTCCTCTGAAAACTCTCTGTTTCTTTAGCTAGATCAAAGATAAAATTCGAAGCT
AAACTCAAGCTAATTCAACCTAAGAAAGAGTTCCTGAATCTTCGGTGAAGTAATATATCA
GATCTGGCATCAAAAACAA
   
Sequence Name 31-K106756-0022-360-C07C-22aq
GenBank Accession LN500181 [GenBank]
Sequencing primer gene specific primer, 22aq: 5'-CAGTATCGATTCGTTTGTTTTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K106756-0022-360-C07C-22aq
TTCCCGAGATCAGGAACTCTTTCTTAGGTTGAATTAGCTTGAGTTTAGCTTCGAATTTTA
TCTTTGATCTAGCTAAAGAAACAGAGAGTTTTCAGAGGAAGAAGAAACAGAGAGTTTTCA
GAGGAAGAAGAAACAGAGAGTAGTGATGACATCAGGGACGAGAATGCCGACATGGAGGGA
AAGAGAGAACAACAAGAGAAGAGAAAGACGACGGAGAGCAATCGCAGCTAAGATCTTCAC
CGGATTAAGAATGTACGGTAATTACGAGCTTCCGAAGCATTGCGACAACAACGAAGTGCT
TAAAGCACTCTGTAACGAAGCTGGTTGGATCGTCGAACCTGATGGAACTACTTACCGCAA
GGTTTGTCTCTCGAATCCGGATCTTAGTTTTGTTCTCTACGCTTCAAGTACATGAAAGTT
TTGAATTTTGGATACAAAATCATTCAGTTTTTGTTAATGATTCAATATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
Primers used for PCR 45wp - 8474, show primer details
   
Sequence Name 44-K118893-0022-360-C07C-45wp
GenBank Accession LN505351 [GenBank]
Sequencing primer gene specific primer, 45wp: 5'-CGCTCTACAGGTCTACTACATCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K118893-0022-360-C07C-45wp
TCATGTGACGTAATCCTTCTAATTCATGAATTACCAAGAAATTACTCTGTTAATAATGAC
AAGGAAGTTACAAGTAGAACTAGTTTTGATTCTATCATTGTGAAAACAAGTGAAAGAGAC
AGTGATCAGGTCAAGGATTCTTGAACCGACAGATTATCTATCATTGGACTCGATTCTCGA
AGTTTGAGTAAAAAGACTTGAGACATAAAAGCAAAAGTGCCAACTTGCTTTCCCAAAAAA
TCTACAGATCAAAACGTACACTTTGAATTCTGTGCAAGAGGACATTATTTTTATCAGTTT
TTATTGAGATCTATGCTACTTTCTTTTTTTGTGGTGAGTATCATCTGAGAATAGACTCGA
CCTGGACAACAAGTGAATTAGTCACACACACACACCATGAAACAGAGAGAGAAAGAATCT
TTTTTTAGAGAAACGCATAATAAAAAGTCACACATAAAGAATCTAACATTTGACTAACGC
TCCTCTAAGCTAAGGCTAGCTAACCTACGCGTATGTCAATCCGAAGTAATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATATAGATGTCCGCAGCG
   
Sequence Name 04-K118892-0022-360-C07C-oriL
GenBank Accession LN488127 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K118892-0022-360-C07C-oriL
TTTACATTGAATTACTTCGGATTGACATACGCGTAGGTTAGCTAGCCTTAGCTTAGAGGA
GCGTTAGTCAAATGTTAGATTCTTTATGTGTGACTTTTTATTATGCGTTTCTCTAAAAAA
AGATTCTTTCTCTCTCTGTTTCATGGTGTGTGTGTGTGACTAATTCACTTGTTGTCCAGG
TCGAGTCTATTCTCAGATGATACTCACCACAAAAAAAGAAAGTAGCATAGATCTCAATAA
AAACTGATAAAAATAATGTCCTCTTGCACAGAATTCAAAGTGTACGTTTTGATCTGTAGA
TTTTTTGGGAAAGCAAGTTGGCACTTTTGCTTTTATGTCTCAAGTCTTTTTACTCAAACT
TCGAGAATCGAGTCCAATGATAGATAATCTGTCGGTTCAAGAATCCTTGACCTGATCACT
GTCTCTTTCACTTGTTTTCACAATGATAGAATCAAAACTAGTTCTACTTGTAACTTCCTT
GTCATTATTAACAGAGTAATTTCTTGGTAATTCATGTAATTAGAAGGATTACGTCACATT
GATCATGTTTGTTTGATATGTTTTCAGGGATGTAGTAGACTGNTAGAGCG

Last Updated on 06.12.2018 16:11