Sequences from confirmation amplicon


Confirmation sequences for line 361A09 and hit At3g11120 are shown.
Line ID 361A09
Locus At3g11120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3481154
Primers used for PCR 25we - 8474, show primer details
   
Sequence Name 29-K118578-0022-361-A09C-25we
GenBank Accession LN499492 [GenBank]
Sequencing primer gene specific primer, 25we: 5'-AAGCAAAGCTAACTACTGGACTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K118578-0022-361-A09C-25we
GGGCACTAGACTCTATTCAGGAATCAATAAGACAACTACAAGCATATTCACTTTGTTCCT
ATGATCAACTTCTAAAGCATCCATAGCTATATATTCCCTCCAATCATCAACATCTGAAGC
AATCACCCAAATCTAATCTCAAAATTATAATTCAGAGAATAGTAGAACATCAACATTTGT
AAGATATATAATAGAAAACATAACTTCCATATTAATTTGGTCCAACGAAACATTCAAACT
GAAACTCAAAACAGAGTTGGATTTAACAACGAAAGCAAAACACAAAACCAGGTTTGTTCG
AAAATCTACTTAGATCGCTGTCTCATCTTTCGTCTCTTCCTCTTTAATCTCCTCATACGC
TTCTTCTTCCACTGTTACAACCCCAAAAACACCATCATTCCGTATTACCAACTATTATTC
CGTATTAGAGAAAATTCATAATAGAAAACATATACATGTCCTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 13-K118577-0022-361-A09C-8409
GenBank Accession LN492262 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K118577-0022-361-A09C-8409
ACATTGAGGACATGTATATGTTTTCTATTATGAATTTTCTCTAATACGGAATAATAGTTG
GTAATACGGAATGATGGTGTTTTTGGGGTTGTAACAGTGGAAGAAGAAGCGTATGAGGAG
ATTAAAGAGGAAGAGACGAAAGATGAGACAGCGATCTAAGTAGATTTTCGAACAAACCTG
GTTTTGTGTTTTGCTTTCGTTGTTAAATCCAACTCTGTTTTGAGTTTCAGTTTGAATGTT
TCGTTGGACCAAATTAATATGGAAGTTATGTTTTCTATTATATATCTTACAAATGTTGAT
GTTCTACTATTCTCTGAATTATAATTTTGAGATTAGATTTGGGTGATTGCTTCAGATGTT
GATGATTGGAGGGAATATATAGCTATGGATGCTTTAGAAGTTGATCATAGGAACAAAGTG
AATATGCTTGTAGTTGTCTTATTTGATTCCTGGAATAGAGTCTAGTTGGCCCCTTACGGT
ACCAATGCAGTCCAG

Last Updated on 06.12.2018 16:11