Sequences from confirmation amplicon


Confirmation sequences for line 361C10 and hit At1g64210 are shown.
Line ID 361C10
Locus At1g64210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23832635
Primers used for PCR EQ36 - 8474, show primer details
   
Sequence Name 09-K103004-0022-361-C10C-8409
GenBank Accession LN490131 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K103004-0022-361-C10C-8409
TCCTGTTGGTCTAATTCTGCTTGACTCACTGGTGATTTCCCTGTCAAGAGCTCCCGTAAT
ACCACACCGAAGCTGTATACATCCCAAAACTGTGTGCTTCTTCTTGTGTCTGTTATTTCA
GGTGCATGGTAGCCAGAAGTAAGGCAAGTGGTTTGAGGAAGTGATCTCATGATTGTCGTC
AAGCCAACATCGCCNATGCAGCCNTAGCATTGTGAGTCTAAGAAGATGTTTGAGGATTTG
ATGTTTCCATGTATGAACTTCCCTTCGTGGATTTTAGCTAGCCCTCTCGCTGCACCGGTT
GCTATTCGCAGTCTAGCATCCCAATCTAACGGTACTCGATGATACCTTCCTCTGTTTCCT
GCAAAGAAAATGGAACCTTGTAGTTTCGATTTATCATCAGAATGAGAGCAATGAAAATAA
CTAACCGTGCAGTATTTCGAAGAGGCTTCCATGATTGTAATAGCTATNAACCGCGAGCTT
ATCGTCTTTGGAGTAGTAGTAAGCCTTTAGCTCAGCTACCTTTTCATGTCTGATCATACC
AATGATCTCCATTTGTTGCTCAAACTCTCGTCTTCCAACGACNACTTCCTTCAATCGTTT
TACAACGACCGTGCTCATGTCCTCCATTGTTACCTTATAGGTTGTACCAAAAGCACC
   
Sequence Name 57-K103005-0022-361-C10C-EQ36
GenBank Accession LN509383 [GenBank]
Sequencing primer gene specific primer, EQ36: 5'-GGGTGCTTTTGGTACAACCTATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K103005-0022-361-C10C-EQ36
ACGATTGAAGGAAGTTGTCGTTGGAAGACGAGAGTTTGAGCAACAAATGGAGATCATTGG
TATGATCAGACATGAAAATGTAGCTGAGCTAAAGGCTTACTACTACTCCTAAGACGATNA
GCTCGCGGTTTATAGCTATTACAATCATGGAAGCCTCTTCGAAATACTGCACGGTTAGTT
ATTTTCATTGCTCTCATTCTGATGATAAATCGAAACTACNAGGTTCCATTTTCTTTGCAG
GAAACAGAGGAAGGTATCATCGAGTACCGTTAGATTGGGATGCTAGACTGCGAATAGCAA
CCGGTGCAGCGAGAGGGCTAGCTAAAATCCACGAAGGGAAGTTCATACATGGAAACATCA
AATCCTCAAACATCTTCTTAGACTCACAATGCTATGGCTGCATAGGCAATGTTGGCTTGA
CGACNATCATGAGATCACTTCCTCAAACCACTTGCCTTACTTCTGGCTACCATGCACCTG
AAATAACAGACACAAGAAGAAGCACACAGTTTTCGGATGTATACNGCTTCGGTGTGGTAT
TACTGGAGCTCTNGACAGGGAAATCACCAGTGAGTCAAGCAGAATTAGTACCAACAGGAG
GTGAGAACATGGACTTAGCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCAGCGTTNTN
ATNANATGAAAGTACATTTTGATAAAACGACNAATTACGATCCGTCGTATTTATNGGCGA
AAGCAATAAACA
Primers used for PCR KF47 - 8474, show primer details
   
Sequence Name 84-K118656-0022-361-C10C-KF47
GenBank Accession LN514479 [GenBank]
Sequencing primer gene specific primer, KF47: 5'-GCATGAACTCAGATCCTAAACCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K118656-0022-361-C10C-KF47
GTTTATCACAAATTGGATCTTCTTCGTATGTTCTTCTTAGATGCTTTTACTTTTTTTCCC
AAATTTGTTCTGTTTTCTTCGTTTTATGAAATGGATTTATATTTGTTTCTGATTCTATAA
TTCAAAGTCTCATTCTTTATTGCATGATTCAAATTCAATAAAATCAAATGATCTATGAAG
AAACAAAGAATATTGCTTTAATGGTTACTACTACTAATTTGTGTTGTCTCACTCTGCATC
AACTGATAGAATATCGTCAATCAATTTCAAAACTAGAGCTATATGAGGTCTTTCTTGTTG
CTTCACAGCAACACATGCCAATCCTATTTGCAACATCTCAACCATCTCCGCCTCGAAACC
ACCTGATTGACTCAATATCTCCGTATCATACACTTCTCCAGACCACTCTTAAGGCACCAC
AGATCTCATCC
   
Sequence Name 58-K118655-0022-361-C10C-8409
GenBank Accession LN509837 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K118655-0022-361-C10C-8409
ATTTCGAAGAGGCTTCCATGATTGTATTAGCTATAAACCGCGAGCTTATCATCTTAGGAG
TAGTAGTAAGCCTTTAGCTCAGCTACATTTTCATGACTGATCGTACCAATGATCTCCATT
TGTTGCTCAAACTCTCATCTTCCAACGACAACTTCCTTCAATCGTTTTACAACGACCGTG
CTCATGTCCTCCATTGTTACCTTATAGGTTGTACCAAAAGCACCCTTCCCCAAAACTTCT
AAAACGAAGAAAACAGAACAAATTTGGGAAAAAAAGTAAAAGCATCTAAGAAGAACATTA
CGAAGAAGATCCAATTTGTGGATTAAACGGGAGAGAGGAGGTGAAAAAGATGAGGTTTAG
GATCTGA

Last Updated on 06.12.2018 16:11