Sequences from confirmation amplicon


Confirmation sequences for line 361E12 and hit At1g29590 are shown.
Line ID 361E12
Locus At1g29590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10340027
Primers used for PCR MJ59 - 8474, show primer details
   
Sequence Name 22-K029910-022-361-E12C-8409
GenBank Accession LN495987 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K029910-022-361-E12C-8409
CTTACAGGGAAAATAACTGTCAAGCGAGCGAGAGAGAAAGAGAAATCAGAAATGGTGGTT
ATGGATTCTCCAGTTTCGGGGAGAATGGCTGATCAGAACATCGATCCTAACACCACGACG
AGCCCTAGTCCTATAGAGAAGCATGTTTCGGCGATTAAGGCTATCTCCGGCGACGAGAAA
GCTCCGTCGAAGGAGAAAAAGAACTACGCGTCGAAGAAATCAACTACCGTAATCCAGAAA
TCGCACTGTTTCCAGAATTCTTGGACATTTTGGTTCGATAATCCTTCCTCCAAATCGAAT
CAAGTCATATGGGGAAGCTCTTTGAGATCTTTGTATACATTCGCTACCATCGAAGAGTTC
TGGAGGTGAGATCTTTGTATCTTTCTTGTTGTCAAATTTCTTTGGTCTAAGCTGGAGTGG
ATTTGAAAT
   
Sequence Name 22-K029910-022-361-E12C-MJ59
GenBank Accession LN495988 [GenBank]
Sequencing primer gene specific primer, MJ59: 5'-CTGCCTCAGAACTATTTCAAATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K029910-022-361-E12C-MJ59
AAGATCTCACCTCCAGAACTCTTCGATGGTAGCGAATGTATACAAAGATCTCAAAGAGCT
TCCCCATATGACTTGATTCGATTTGGAGGAAGGATTATCGAACCAAAATGTCCAAGAATT
CTGGAAACAGTGCGATTTCTGGATTACGGTAGTTGATTTCTTCGACGCGTAGTTCTTTTT
CTCCTTCGACGGAGCTTTCTCGTCGCCGGAGATAGCCTTAATCGCCGAAACATGCTTCTC
TATAGGACTAGGGCTCGTCGTGGTGTTAGGATCGATGTTCTGAT

Last Updated on 06.12.2018 16:11