Sequences from confirmation amplicon


Confirmation sequences for line 361F02 and hit At1g79530 are shown.
Line ID 361F02
Locus At1g79530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29918826
Primers used for PCR IW59 - 8474, show primer details
   
Sequence Name 30-K033212-022-361-F02C-IW59
GenBank Accession LN499618 [GenBank]
Sequencing primer gene specific primer, IW59: 5'-CTTTGTCTTTCCACTGCTACTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K033212-022-361-F02C-IW59
CACACAACAGGAAAACACATCCAATAAAACTATTAATTTTCTTACTTACTTCGAACTGCA
GATGGCACTTCTGTAGCCGTGGCCTTGATGGGTTGCACACTTCTTGCATTGCCATTTCTG
TACATTTAAACATAAACAAAACAAACACTCTGAGAATCGAAAAGTGCATCATACATCAAT
AACTTTTACACATGTTACAGAATTTGTCACAGAACTGTAGAGATGAAGATATCCCAGAAG
AAAATCTTGATGGCTTCAGGTTGCGACTAAATCCAAGACTTGACAACACCTGAGAAATTT
GTGACAAAAGATAATAAATACGATTGTTTTCTTGCAAAGTTCCTGGAAAAACTTAAATAA
AAAAACAAACAACTCCAAATGTATTGCCATTAGACATTCTAAAAGAGCAACAAATAAGAT
TTGAAACCTCACAAGTTGAAATTCATCGTTAAACGAGACTAGATACAAAAAACGCAAGAA
CGAAGACAAAAATAGGATTAAACGTCACAAGCTTACGCTAAGAAACTCAGAACAACTTCC
AAGTAATATTTATCACATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 22-K033212-022-361-F02C-8409
GenBank Accession LN496012 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K033212-022-361-F02C-8409
ATTTACAATTGATGTGATAAATATTACTTGGAAGTTGTTCTGAGTTTCTTAGCGTAAGCT
TGTGACGTTTAATCCTATTTTTGTCTTCGTTCTTGCGTTTTTTGTATCTAGTCTCGTTTA
ACGATGAATTTCAACTTGTGAGGTTTCAAATCTTATTTGTTGCTCTTTTAGAATGTCTAA
TGGCAATACATTTGGAGTTGTTTGTTTTTTTATTTAAGTTTTTCCAGGAACTTTGCAAGA
AAACAATCGTATTTATTATCTTTTGTCACAAATTTCTCAGGTGTTGTCAAGTCTTGGATT
TAGTCGCAACCTGAAGCCATCAAGATTTTCTTCTGGGATATCTTCATCTCTACAGTTCTG
TGACAAATTCTGTAACATGTGTAAAAGTTATTGATGTATGATGCACTTTTCGATTCTCAG
AGTGTTTGTTTTGTTTATGTTTAAATGTACAGAAATGGCAATGCAAGAAGTGTGCAACCC
ATCAAGGCCACGGCTACAGAAGTGCCATCTGCAGTTCGAAGTAAGTAAGAAAATTAATAG
TTTTATTGGATGTGTTTTCCTGTTGTGTGCTTATTGGTGAATTTGATTTGCTTGAGTAGG
GTCAAGTAGCAGTGAAAAGACAAA

Last Updated on 06.12.2018 16:11