Sequences from confirmation amplicon


Confirmation sequences for line 361H10 and hit At4g24810 are shown.
Line ID 361H10
Locus At4g24810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12786845
Primers used for PCR 18uj - 8474, show primer details
   
Sequence Name 73-K117751-0022-361-H10C-18uj
GenBank Accession LN512967 [GenBank]
Sequencing primer gene specific primer, 18uj: 5'-GTGTTGTGAGGGATTTTTGTTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K117751-0022-361-H10C-18uj
ATTCATTCTTTCACTCATTCTCAAATTTGGGTTCGAACCACAACCAAATGTATATTGCAA
ATGCGGTTTAAAGCTACTTCTGGTTCTTGATGATGATACTCTCACAAATTTGACTATTAT
AGTTCCTAGTAGTTGCCATGTAACTTTGTCGGTGTATCTCACAAATAGCACAAAAGTAGA
GTTTTAAAACTTACGCAACTCTCCTTGTGCTCAAATCTGACATTATAATGACAAAACTTT
TATAAAGATGTACTCACTAGTCAAAGTTTCAAACCAACTAAACCGGTTAAGGTAAGAGAC
AACTTGCTGCCTTAGCTGTATCCAGAAAATAATGTAAAGACTAAAGACTGAGAGCATCAT
TTGCAAGAGGCAGGGGATTTTATTAACTCTCTCTTGGCCCTGGGTATAGCCTTCTAAGAG
AACTACATCGACGCTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATTC
   
Sequence Name 48-K117750-0022-361-H10C-8409
GenBank Accession LN506628 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K117750-0022-361-H10C-8409
AATATATCCCTGAAGCGTCGATGTAGTTTCTCTTAGAAGGCTATACCCAGGGCCAAGAGA
GAGTTAATAAAATCCCCTGCCTCTTGCAAATGATGCTCTCAGTCTTTAGTCTTTACATTA
TTTTCTGGATACAGCTAAGGCAGCAAGTTGTCTCTTACCTTAACCGGTTTAGTTGGTTTG
AAACTTTGACTAGTGAGTACATCTTTATAAAAGTTTTGTCATTATAATGTCAGATTTGAG
CACAAGGAGAGTTGCGTAAGTTTTAAAACTCTACTTTTGTGCTATTTGTGAGATACACCG
ACAAAGTTACATGGCAACTACTAGGAACTATAATAGTCAAATTTGTGAGAGTATCATCAT
CAAGAACCAGAAGTAGCTTTAAACCGCATTTGCAATATACATTTGGTTGTGGTTCGAACC
CAAATTTGAGAATGAGTGAAAGAATGAATGAAATTCATTTATTTCCATGTCAACTTTTCA
ATAATCACAGAAAAAAAACAAA

Last Updated on 06.12.2018 16:11