Sequences from confirmation amplicon


Confirmation sequences for line 362A03 and hit At1g68020 are shown.
Line ID 362A03
Locus At1g68020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25498249
Primers used for PCR FQ75 - 8474, show primer details
   
Sequence Name 34-K033882-022-362-A03C-8409
GenBank Accession LN501320 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K033882-022-362-A03C-8409
TGAAGCCATTTACAATTGAATATANTCCTGCTTCATATCATACTTTGAATAGGCAAAGTT
TTGTAAATCTCAGACGATGGAAATGGACTATGAAGGAAGAAACCAAGCTTGACTCTGTTA
AACCTCTTCCTCAAGAAAGTAGGCAAAACCATCAAATGATAATCATGTATCCACACAAAA
TCATCCTCAGGATTAATCACCTCCATAATTCTATCAGCAAAAATCTTGTTCACAGAGACA
TAAGCTTGCCACAAAGTCCTATCAAACCTACCTCCAAGATCAGGAGAAAGTGGCAACATG
TAATGAAACAAAGGCCAAAGCTGTTGCTTACAGAACCCATGGTA
   
Sequence Name 90-K033882-022-362-A03C-FQ75
GenBank Accession LN515002 [GenBank]
Sequencing primer gene specific primer, FQ75: 5'-CTACCATGGATTCTGTAAGCAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K033882-022-362-A03C-FQ75
GTTTCTTACATGTTGCACTTTCTCCTGATCTTGGAGGTAGGTTTGATAGGACTTTGTGGC
AAGCTTATGTCTCTGTGAACAAGATTTTTGCTGATAGAATTATGGAGGTGATTAATCCTG
AGGATGATTTTGTGTGGATACATGATTATCATTTGATGGTTTTGCCTACTTTCTTGAGGA
AGAGGTTTAACAGAGTCAAGCTTGGTTTCTTCCTTCATAGTCCATTTCCATCGTCTGAGA
TTTACAAAACTTTGCCTATTCAAAGTATGATATGAAGCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTC
CCAAATGG

Last Updated on 06.12.2018 16:11