Sequences from confirmation amplicon


Confirmation sequences for line 362B02 and hit At1g13950 are shown.
Line ID 362B02
Locus At1g13950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4773647
Primers used for PCR 06hk - 8474, show primer details
   
Sequence Name 43-K112551-0022-362-B02C-06hk
GenBank Accession LN504889 [GenBank]
Sequencing primer gene specific primer, 06hk: 5'-CCCAATTCAACAAAAACAAAATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K112551-0022-362-B02C-06hk
ACAGAATGAAGTGATCAAAGGAGCTTTTTTCACATACATCACAATTGTGGGAAGAAGGAA
CAATATCTTCGAGTTTCTTGCTGGTGAAGATATCAATAGCTACAAAATGACATTTAGCAT
GACCATGCTTGCCAGTCTTCGAGGTTGAAACCTCAACAACCTGCGACACAAAATGACATT
TAGCAGCTGATCCAGTAACTTGTGTAAGAAAAAATCCTAAAACTTATCTAATCGAACAAA
ACTGAAGATGACCAATTTAATAATTTCGCTAAAACAAAAGTGCAATAAAATGTGAATTTA
ACAATGATGACAAGAACAAATATCAGTCGAACATCGTGGAGGTGACAAAAAAAAGAATCT
GAGGAATCTAAGTAATGAACAGATCTAATAAGATCAGAGGAAGAGAGTGGAGAAATGTTT
GAGAACGAAACCTTGCAGGGACGATTTTTGATGACGATGTAACCATTCTTACGGATGGTT
CCAGCTTGTTGAGGGTAGGTTTTGGACGCTCCGGCGTCACTGGACTCAAAGTGATGCTCC
TCGTCGGACATGGTTGCAGCAGCAATATGGAGAAACAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 19-K112550-0022-362-B02C-8409
GenBank Accession LN494826 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K112550-0022-362-B02C-8409
ATCACTTTGAGTCCAGTGACGCCGGAGCGTCCAAAACCTACCCTCAACAAGCTGGAACCA
TCCGTAAGAATGGTTACATCGTCATCAAAAATCGTCCCTGCAAGGTTTCGTTCTCAAACA
TTTCTCCACTCTCTTCCTCTGATCTTATTAGATCTGTTCATTACTTAGATTCCTCAGATT
CTTTTTTTTGTCACCTCCACGATGTTCGACTGATATTTGTTCTTGTCATCATTGTTAAAT
TCACATTTTATTGCACTTTTGTTTTAGCGAAATTATTAAATTGGTCATCTTCAGTTTTGT
TCGATTAGATAAGTTTTAGGATTTTTTCTTACACAAGTTACTGGATCAGCTGCTAAATGT
CATTTTGTGTCGCAGGTTGTTGAGGTTTCAACCTCGAAGACTGGCAAGCATGGTCATGCT
AAATGTCATTTTGTAGCTATTGATATCTTCACCAGCAAGAAACTCGAAGATATTGTTCCT
TCTTCCCACAATTGTGATGTATGTGAAAAAAGCTCCTTTGATCACTTTCATTTCTTGTTT
GTTTCTTTCAAGTCCCATTTGAGATTTTGTTTT

Last Updated on 06.12.2018 16:11