Sequences from confirmation amplicon


Confirmation sequences for line 362D09 and hit At4g32330 are shown.
Line ID 362D09
Locus At4g32330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15611514
Primers used for PCR GE22 - 8474, show primer details
   
Sequence Name 10-K033717-022-362-D09C-GE22
GenBank Accession LN490552 [GenBank]
Sequencing primer gene specific primer, GE22: 5'-AAAAGAGGTTAGCATTCTGGTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K033717-022-362-D09C-GE22
CAAAAATTACTGTAATCATATATCTGCTTTCAATGGTGCAGTTCTATGTTACGCTTGAGG
AGAAGACTCATGCAAAAGAAGAGGAAATTAATAGCATGCAAGCCAAGTCCAAGGTTGTCT
CCTATACCCACCCTGTTTATTGTCATAGTGTTTAGATGCTTGGCCTCCTCCAAAACAAAA
TTCTTGGTTTCTCGCAGGAAACACAAGAAGCTGAGCTTAGGATGCTAAGGAAGAGTTTGA
ACTTCAAAGCCACACCTATGCCTAGCTTTTATCAAGAACCTCAGCCGCCTAAAACAGAGT
TAAAGAAGGTACTTCTGCTCTATGCTCAAACCTGATCTCACTTAATATTAAGTACTAATA
TTCACGTTTCTTGTAAAAATTTTGTTCACGTCCAAGAAGATACCACCAACGAGACCAAAA
TCTCCAAAACTCGGGCGAAAGAAGACTGCTTCTGGAGCAGATTCTGAAGAAACTCAAACC
CCTCGCCTTGGTAGGCTAAGCCTAGATGAGAGAGCTTCTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAAAATGGG
   
Sequence Name 90-K033717-022-362-D09C-8409
GenBank Accession LN515001 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K033717-022-362-D09C-8409
AAGCTCTCTCATCTAGGCTTAGCCTACCAAGGCGAGGGGTTTGAGTTTCTTCAGAATCTG
CTCCAGAAGCAGTCTTCTTTCGCCCGAGTTTTGGAGATTTTGGTCTCGTTGGTGGTATCT
TCTTGGACGTGAACAAAATTTTTACAAGAAACGTGAATATTAGTACTTAATATTAAGTGA
GATCAGGTTTGAGCATAGAGCAGAAGTACCTTCTTTAACTCTGTTTTAGGCGGCTGAGGT
TCTTGATAAAAGCTAGGCATAGGTGTGGCTTTGAAGTTCAAACTCTTCCTTAGCATCCTA
AGCTCAGCTTCTTGTGTTTCCTGCGAGAAACCAAGAATTTTGTTTTGGAGGAGGCCAAGC
ATCTAAACACTATGACAATAAACAGGGTGGGTATAGGAGACAACCTTGGACTTGGCTTGC
ATGCTATTAATTTCCTCTTCTTTTGCATGAGTCTTCTCCTCAAGCTTAACATAGAACTGC
ACCATATGAAAGCAGATATATGATTACATGTAATTATTTTTGTGGCGAGAGGACAATCTA
GTGAACCAGAATGCTAACCTCTT

Last Updated on 06.12.2018 16:11