Sequences from confirmation amplicon


Confirmation sequences for line 362H02 and hit At1g02530 are shown.
Line ID 362H02
Locus At1g02530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:531710
Primers used for PCR 90yp - 8474, show primer details
   
Sequence Name 25-K121846-0022-362-H02C-90yp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 90yp: 5'-GTTGAACAGACAATCGGGTCTATC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K121846-0022-362-H02C-90yp
GAACAATCTTTAAGTAGATTAGTTGCTAAATTCCATTTTTTTTTAAAAGGTTGCTTCTTT
TACGGGAGAGAAGCAAGCAATAAACAGCTACAAGAAGTACATAACATCGGCTTATAAATC
AAGCATTCAACAAGGTTTCTCCACTGGTTTAGGACTTGGAGTAATGATCTATGTGTTCTT
CAGCAGCTATGCTTTGGCTATTTGGTTTGGTGGGAAGATGATACTCGAAAAAGGGTATAC
CGGTGGCTCTGTGATCAACGTGATCATCATTGTGGTTGCGGGTTCAATGTAAATGCCTAG
ATCATTGGTTCTATATATACATCAGATTATGTCTTCTTCTTTGTTTATAAATGTGAGATT
CCTAAGAGTTGTGTTCTGTTTTGTTTTTCTTTAGGTCGCTTGGGCAAACATCTCCATGTG
TAACCGCATTTGCAGCAGGTCAAGCTGCAGCATATAAGATGTTTGAGACGATCAAAAGAA
AGCCTTTGATTGATGCTTATGACGTAAATGGAAAGGTTCTTGGAGATATCCGAGGAGACA
TTGAACTGAAAGATGTGCATTTCAGTTACCCCGCGAGGCCAGACGAGGAGATATTTGATG
GATTCTCTCTGTTTATCCCGAGCGGTGCAACAGCAGCACTGGTAGGAGAAAGTGGAAGTG
GAAAATCTACGGTGATAAATTTGATTGAGAGGTTTTATGATCCGAAAGCCGGTGAAGTAC
TCATTGATGGAATTAATCTTAAGGAGTTTCAGTTGAAATGGATCAGAAGCAAAATCGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
   
Sequence Name 09-K121845-0022-362-H02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K121845-0022-362-H02C-8409
ATTGAATATATCCGATTTTGCTTCTGATCCATTTCAACTGAAACTCCTTAAGATTAATTC
CATCAATGAGTACTTCACCNGGCTTTCGGATCATAAAACCTCTCAATCAAATTTATCACC
GTAGATTTTCCACTTCCACTTTCTCCTACCAGTGCTGCTGTTGCACCGCTCGGGATAAAC
AGAGAGAATCCATCAAATATCTCCTCGTCTGGCCTCGCGGGGTAACTGAAATGCACATCT
TTCAGTTCAATGTCTCCTCGGATATCTCCAAGAACCTTTCCATTTACGTCATAAGCATCA
ATCAAAGGCTTTCTTTTGATCGTCTCAAACATCTTATATGCTGCAGCTTGACCTGCTGCA
AATGCGGTTACACATGGAGATGTTTGCCCAAGCGACCTAAAGAAAAACAAAACAGAACAC
AACTCTTAGGAATCTCACATTTATAAACAAAGAAGAAGACATAATCTGATGTATATATAG
AACCAATGATCTAGGCATTTACATTGAACCCGCAACCACAATGATGATCACGTTGATCAC
AGAGCCACCGGTATACCCTTTTTCGAGTATCATCTTCCCACCAAACCAAATAGCCAAAGC
ATAGCTGCTGAAGAACACATAGATCATTACTCCAAGTCCTAAACCAGTGGAGAAACCTTG
TTGAATGCTTGATTTATAAGCCGATGTTATGTACTTCTTGTAGCTGTTTATTGCTTGCTT
CTCTCCCGTAAAAGAAGCAACCTTTTAAAAAAAAATGGAATTTAGCAACTAATCTTACTT
AAAGATTTGTTCTGTAGCTTAGAAAGACTGATTTTTACCGTTCGGAT

Last Updated on 06.12.2018 16:11