Sequences from confirmation amplicon


Confirmation sequences for line 363A08 and hit At3g51880 are shown.
Line ID 363A08
Locus At3g51880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19247935
Primers used for PCR CP19 - 8474, show primer details
   
Sequence Name 50-K031138-022-363-A08C-CP19
GenBank Accession LN507063 [GenBank]
Sequencing primer gene specific primer, CP19: 5'-CACTGGCTTCATCTTCATCATTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K031138-022-363-A08C-CP19
TCCCTTCCTCCTGTCCCCATTGCAAGGAAATCAGAGTTAGCTATGTACGAGTTTGTATAT
ATGAGAGTAAGGCTTAGGGCTTACCAAGTTTTTGTTGTATGCATCCATTTGCTTCTCATA
TTCAGCTTTCCTTTTTGCAGCTTTCTCTTCATATGGAGCTTTTTCCTTTACAAAGGAAGA
AGTGAAACTCATGTCAATCTCACACTATTTTCCTCACAGTATCAGACAGGGAAGTTTCAA
AGATACTTACAGCTTGAGACATTGACTTCCATTTCTGCCCTCCAGCTTTCCCAACCTTGA
CAAGGAAAAGAGAAGGTGAGACCATGAGGACTTCACCTAGTAAAGACTACCGTACATGTA
AACTATCAAGGAATCTGCCAAATGCTTACAGCAGAGACGGCCTTCACATTTGGATTTTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
ACTTC
   
Sequence Name 15-K031138-022-363-A08C-8409
GenBank Accession LN492851 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K031138-022-363-A08C-8409
ATTTACAATTGAAAATCCAAATGTGAAGGCCGTCTCTGCTGTAAGCATTTGGCAGATTCC
TTGATAGTTTACATGTACGGTAGTCTTTACTAGGTGAAGTCCTCATGGTCTCACCTTCTC
TTTTCCTTGTCAAGGTTGGGAAAGCTGGAGGGCAGAAATGGAAGTCAATGTCTCAAGCTG
TAAGTATCTTTGAAACTTCCCTGTCTGATACTGTGAGGAAAATAGTGTGAGATTGACATG
AGTTTCACTTCTTCCTTTGTAAAGGAAAAAGCTCCATATGAAGAGAAAGCTGCAAAAAGG
AAAGCTGAATATGAGAAGCAAATGGATGCATACAACAAAAACTTGGTAAGCCCTAAGCCT
TACTCTCATATATACAAACTCGTACATAGCTAACTCTGATTTCCTTGCAATGGGGACAGG
AGGAAGGGAGTGATGAATCTGAAAAGTCTAGATCTGAGATAAATGATGAAGAAAGGGCCA
GTG

Last Updated on 06.12.2018 16:11