Sequences from confirmation amplicon


Confirmation sequences for line 363C03 and hit At1g07340 are shown.
Line ID 363C03
Locus At1g07340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2256504
Primers used for PCR 25sa - 8474, show primer details
   
Sequence Name 45-K116321-0022-363-C03C-25sa
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 25sa: 5'-AGATGACCGCTACACAGGTTAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K116321-0022-363-C03C-25sa
AAAACCAACTTTGTTTTTTTTTGAACCCCCTACCCAAAAAAAAATTGGGGGGTTTTCTTT
ACCCCCATTAAAACCATTTGGGCCAAATAAGGGCCCAGCCCGGCCCCTAAATTTTTTTAC
CCCCCCCCGGCTAAACCTTTTTGGATTTTCAGGGCCTTGGGGCCCCTTGGGAAGGGTTTT
TCCCCCAAAAAACCCCCCCCTTAAAAGAAAAAAAGGGGGTTACTTTTGGGGGGGGGGAAA
AAAAAATTTCAATTTGAAAGGGCTTCCTGCCCGGGAAAACCCCCGGGGACCACCAAGAAT
TAAAGGGGCAAATTGGAAAAAAAAAAAAAATAATTCCCCATTTTTTTTCAATTCAAAAAG
GGAAATTCCCC
   
Sequence Name 13-K116320-0022-363-C03C-8409
GenBank Accession LN492204 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K116320-0022-363-C03C-8409
ATTGAATATGTTCATCGCCACCGCACAGAAGTAACCAGCATTTCTCACTTCAAGAGGGTA
GATCTCAGACGGAACAAGCCATCCCAATGGACCCCAAGACCATGCAAATCCAGACACGTA
TACGCAGATGAGGATCAATACAATCAGCGGCACGGCATGGCCAGTAATAGGACCAACTAG
CTTCAAGTGAGCTAAGAGAATGCCTCCAATTGTCATCTGCGTAAGATGATCAAAAAAAAT
ACAAGTTAGCTTGTTATCCAAGTAAGAATATGTGTACCATGTGACACAAGCAAAAAAAAA
AAAAAAAAACCACCCGGG

Last Updated on 06.12.2018 16:11