Sequences from confirmation amplicon


Confirmation sequences for line 363D03 and hit At1g67250 are shown.
Line ID 363D03
Locus At1g67250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25164010
Primers used for PCR RP01 - 8474, show primer details
   
Sequence Name 26-K018976-022-363-D03C-RP01
GenBank Accession LN497711 [GenBank]
Sequencing primer gene specific primer, RP01: 5'-TCTTGAATCTCTGTTGATTCATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K018976-022-363-D03C-RP01
AGGAAAAGAAATCGAAAGAGGCGTTGAAGAGGACGGTCATTGCGCATGCCTACGGGACTG
CACTTCCGTTGAAGATGGATATGGATAGGCAAATTCTCTCAAGGTAAGCTTCTGCTAATA
CTTCTTTGTACTGGAACACTCAGATTTACATGAAATGTGTGAATCAATGCATTGAGAAGC
TATTCTATGTTGCTTAGTAAGATGCACACACTTAATAGGTCCCAATTATGTATAATGGTT
GTGTGCATTAGTGGTATCTATTGTGTACTCAAAACTATCTGTGTGTATCATTGAGCTCAG
GGTCTAAACTGTTTGCTGTATTATCTGCTGGTTGTTAATAGCACTATGAAAATAAGTTGT
ATAGTTCTCAATTTTTTCTTTTTCTCTGGAATATTTAGGTTTCAGAGACCTCCTGGACCA
ATTCCGTCGTCGATGCTCGGTTTAGAAGTCTACACGGGAGCTGTGGATGACTTTGGTTTT
GAGGATTACTTGAATGGTAAATGTCTTTTACTCCAATTGCAAGTGTCTCAATGCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCT
   
Sequence Name 30-K018976-022-363-D03C-8409
GenBank Accession LN499527 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K018976-022-363-D03C-8409
CATGTAGATTTCCCGGACATGCATTGAGACACTTGCAATTGGAGTAAAAGACATTTACCA
TTCAAGTAATCCTCAAAACCAAAGTCATCCACAGCTCCCGTGTAGACTTCTAAACCGAGC
ATCGACGACGGAATTGGTCCAGGAGGTCTCTGAAACCTAAATATTCCAGAGAAAAAGAAA
AAATTGAGAACTATACAACTTATTTTCATAGTGCTATTAACAACCAGCAGATAATACAGC
AAACAGTTTAGACCCTGAGCTCAATGATACACACAGATAGTTTTGAGTACACAATAGATA
CCACTAATGCACACAACCATTATACATAATTGGGACCTATTAAGTGTGTGCATCTTACTA
AGCAACATAGAATAGCTTCTCAATGCATTGATTCACACATTTCATGTAAATCTGAGTGTT
CCAGTACAAAGAAGTATTAGCAGAAGCTTACCTTGAGAGAATTTGCCTATCCATATCCAT
CTTCAACGGAAGTGCAGTCCCGTAGGCATGCGCAATGACCGTCCTCTTCAACGCCTCTTT
CGATTTCTTTTCCTGAAACCGATGAATCA

Last Updated on 06.12.2018 16:11