Sequences from confirmation amplicon


Confirmation sequences for line 363E06 and hit At3g49620 are shown.
Line ID 363E06
Locus At3g49620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18393837
Primers used for PCR 07gm - 8474, show primer details
   
Sequence Name 44-K111955-0022-363-E06C-07gm
GenBank Accession LN505227 [GenBank]
Sequencing primer gene specific primer, 07gm: 5'-AATGCTCAAACTCTTATCAAGGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K111955-0022-363-E06C-07gm
TATTATTTACTTATCCTCCAACTAACTTACACGTGATGATAAACATTTGTATAATCTTTG
GTAATTACTTTTTATCTAAAATGCGAATAAACTTTTAAAAATGTATTCAACAAACCAATC
ACAAAGCATTAAAGCTACTAAGAAATTACTAATTTTATTTTAAAAAGTTATAAAACCGAA
TAATAACGATCACTACAGTTTATTTGAGTGACAAAAATATATGTCTGATAACAGATCACT
CTATGATTTCCCACGTGGGCTGCTCCGTTTGGCCTTTAGAAATCCTAAGGGACAATTTTT
AATGTGTATTTATACAAATGAGATAACCATTTATGGTAATATATCATTTGCGATGGATCA
GCAATTACCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATA
   
Sequence Name 36-K111954-0022-363-E06C-8409
GenBank Accession LN502274 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K111954-0022-363-E06C-8409
GAGCCTTTACATTGGTAATTGCTGATCCATCGCAAATGATATATTACCATAAATGGTTAT
CTCATTTGTATAAATACACATTAAAAATTGTCCCTTAGGATTTCTAAAGGCCAAACGGAG
CAGCCCACGTGGGAAATCATAGAGTGATCTGTTATCAGACATATATTTTTGTCACTCAAA
TAAACTGTAGTGATCGTTATTATTCGGTTTTATAACTTTTTAAAATAAAATTAGTAATTT
CTTAGTAGCTTTAATGCTTTGTGATTGGTTTGTTGAATACATTTTTAAAAGTTTATTCGC
ATTTTAGATAAAAAGTAATTACCAAAGATTATACAAATGTTTATCATCACGTGTAAGTTA
GTTTGGAGGATAATGATAAATAAATTAAAAAGTGAGTGATCCAAATCCCTTGATAAGAGT
TTGAGCATTATCCTCCAAACTAACTTACACGTGATGATAAACATTTGTATAATCTTTGGT
AATTACTTTTTATCTAAAATGCGAATAAACTTTTAAAAATGTATTCAACAAACCAATCAC
AAAGCATTAAAGCTACTAAGAAATTACTAAT

Last Updated on 06.12.2018 16:11