Sequences from confirmation amplicon


Confirmation sequences for line 365C11 and hit At1g04580 are shown.
Line ID 365C11
Locus At1g04580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1255116
Primers used for PCR CD84 - 8474, show primer details
   
Sequence Name 34-K105566-0022-365-C11C-CD84
GenBank Accession LN501416 [GenBank]
Sequencing primer gene specific primer, CD84: 5'-ATTGGAAGTGCATTGCCTTATATC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K105566-0022-365-C11C-CD84
CATCAAGCGGCATCATTGTGGATAAATGCCGGTTGGCATTTGGTTCTTATGGTGGGTATC
ATTCCATCAGGGCGAGGGAAGTTGAGGATTTCTTGACGGGTAAAATACTCAGCCACAGTG
TTTTATATGAAGCTGTGAGGTTACTTAAAGGGATCATAGTTCCCAGCATAGATACCTCAT
ATTCTGAGTATAAGAAAAGCTTGGCCGTTGGGTTTCTCTTTGATTTTCTCTATCCTCTGA
TCGAGAGTGGCAGTTGGGATTCCGAAGGAAAGCATATCGATGGGCACATCGATCCAACAA
TATGTCTACCTTTGCTGTCATCTGCGCAGCAGGTGTTTGAAAGTAAAGAATATCACCCTG
TTGGTGAAGCCATTATCAAATTTGGAGCTGAAATGCAGGCTTCTGGTTAGTACTATATTT
TTCGTTGGATCGTTTTTAAATACCTTTATATTTTCAATGCTGTGAAAAGGCTCAAGCAGC
AATGCTTTTAGTTCTTTAGTCATATGCTTATTTAGCTAAGTAATATCAGACCTTACAGGA
GCGTTTATCTTTCCAGGAGCGTTTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 66-K105567w-0022-365-C11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K105567w-0022-365-C11C-8409
GCCATTTACATTGAAAAACGCTCCTGGAAAGATAAACGCTCCTGTAAGGTCTGATATTAC
TTAGCTAAATAAGCATATGACTAAAGAACTAAAAGCATTGCTGCTTGAGCCTTTTCACAG
CATTGAAAATATAAAGGTATTTAAAAACGATCCAACGAAAAATATAGTACTAACCAGAAG
CCTGCATTTCAGCTCCAAATTTGATAATGGCTTCACCAACAGGGTGATATTCTTTACTTT
CAAACACCTGCTGCGCAGATGACAGCAAAGGTAGACATATTGTTGGATCGATGTGCCCAT
CGATATGCTTTCCTTCGGAATCCCAACTGCCACTCTCGATCAGAGGATAGAGAAAATCAA
AGAGAAACCCAACGGCCAAGCTTTTCTTATACTCAGAATATGAGGTATCTATGCTGGGAA
CTATGATCCCTTTAAGTAACCTCACAGCTTCATATAAAACACTGTGGCTGAGTATTTTAC
CCGTCAAGAAATCCTCAACTTCCCTCGCCCTGATGGAATGATACCCACCATAAGAACCAA
ATGCCAACCGGCATTTATCCACAATGATGCCGCTTGATGATGCATCGTGAGAGACTACTG
CTAAGAAAGCAGCGTTGATATAAGGC
Primers used for PCR CD85 - 8474, show primer details
   
Sequence Name 32-K118837-0022-365-C11C-CD85
GenBank Accession LN500775 [GenBank]
Sequencing primer gene specific primer, CD85: 5'-GTGTCTGCAACCTGATAGGTCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K118837-0022-365-C11C-CD85
AAGTTGATAGAGATGCAGGAATTATTTCGGTGGTGGATGGATTGGGAATGTATTAGAAAT
TGGACTTACCACAAGAGCAATTATTTGTCCGGCAGATATAGTAACTTCATCTGCAAATAG
AAGCCCTGTGCCAAACATCGTTATGTAACCAATGTTTTGCCCCACTTCAGGTATATCTTT
GAAAGTAATAACTGCCAAAACTCCAATTGGTGTCACATTTCCTGAGAAACCAACGCTCTT
TATCCAAGCCAATGGCTTTGTGCTATAAATGAATGCTCCATGTAGACAATGGGGTAAGGA
TGGAATATCATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATATAACACAACGTTATTA
   
Sequence Name 08-K118836-0022-365-C11C-8409
GenBank Accession LN489947 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K118836-0022-365-C11C-8409
CATTTACATTGATGATATTCCATCCTTACCCCATTGTCTACATGGAGCATTCATTTATAG
CACAAAGCCATTGGCTTGGATAAAGAGCGTTGGTTTCTCAGGAAATGTGACACCAATTGG
AGTTTTGGCAGTTATTACTTTCAAAGATATACCTGAAGTGGGGCAAAACATTGGTTACAT
AACGATGTTTGGCACAGGGCTTCTATTTGCAGATGAAGTTACTATATCTGCCGGACAAAT
AATTGCTCTTGTGGTAAGTCCAATTTCTAATACATTCCCAATCCATCCACCACCGAAATA
ATTCCTGCATCTCTATCAACTTTCTCGTCTTTCTTCTAACTCTTAGTCCATTTTTTATGA
CCTATCAGGTTGCAGACA

Last Updated on 06.12.2018 16:11