Sequences from confirmation amplicon


Confirmation sequences for line 365D09 and hit At1g76810 are shown.
Line ID 365D09
Locus At1g76810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28834788
Primers used for PCR 07ey - 8474, show primer details
   
Sequence Name 20-K111225-0022-365-D09C-07ey
GenBank Accession LN495262 [GenBank]
Sequencing primer gene specific primer, 07ey: 5'-AATTCTTTCTTAACCACAGGCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K111225-0022-365-D09C-07ey
ATCACCTTTAGGTTAGATCGACCGTACCCCAGCTTTTCGCATCCCATTCATCTTCTTCGT
CATCTTCCTCAACCCCATTTTCCTGAGCTACATCAGCAGGTCCTGAATTTTCGTCTGTGT
TTACTAACTCTATTAAATCAACCTTTCCAGTGTCTGTTAAACCCACTTCACCTAAAGTAT
CTTGTTCATCTGCTTGATTCTCTTTTGGCTCTACCTCATCTTCCCCCTGAACAGAAGTGT
CAATGCCTTTCTGGCGAGATGATTTTTTCTTGTTGGCGTAAATGGGACGCTTTGAGGAAG
TAGCATCACCATCATTATCTGCAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAT
   
Sequence Name 04-K111224-0022-365-D09C-8409
GenBank Accession LN487974 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K111224-0022-365-D09C-8409
CAGATAATGATGGTGATGCTACTTCCTCAAAGCGTCCCATTTACGCCAACAAGAAAAAAT
CATCTCGCCAGAAAGGCATTGACACTTCTGTTCAGGGGGAAGATGAGGTAGAGCCAAAAG
AGAATCAAGCAGATGAACAAGATACTTTAGGTGAAGTGGGTTTAACAGACACTGGAAAGG
TTGATTTAATAGAGTTAGTAAACACAGACGAAAATTCAGGACCTGCTGATGTAGCTCAGG
AAAATGGGGTTGAGGAAGATGACGAAGAAGATGAATGGGATGCGAAAAGCTGGGGTACGG
TCGATCTTAACCTTAAAGGTGATTTTGATGACGAGGAGGAAGAAGCTCAGCCTGTGGTTA
AGAAAGAAT

Last Updated on 06.12.2018 16:11