Sequences from confirmation amplicon


Confirmation sequences for line 365E12 and hit At2g19190 are shown.
Line ID 365E12
Locus At2g19190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8326768
Primers used for PCR IS01 - 8474, show primer details
   
Sequence Name 31-K024207-022-365-E12C-IS01
GenBank Accession LN499994 [GenBank]
Sequencing primer gene specific primer, IS01: 5'-TCATAAATGGAGAACAAGTTGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K024207-022-365-E12C-IS01
AAGAATCAGCTCAAGGCTACAAAGAGTTTCGAGCAGAGGTTTTTTTTGCGTACCTTTCAA
GGACTTAATCTTAATTTATCTCAAACTTTTTCACCTAACTAATCTAGTTGAGAATCGCAG
GTTGACCTTCTCATGAGAGTTCATCACACGAACCTGACTTCTCTTGTTGGATATTGCAAC
GAAATAAACCACATGGTGCTTATCTATGAGTATATGGCTAATGAGAACTTAGGAGACTAT
TTGGCAGGTTAATTAATGATTCTTCTTTTGTTCACTAATCTTATGTTCATAATGTACTGA
GAAAGGAACTTTGGTTTTGTTTCATTTTGTGAAGGTAAAAGGTCATTTATCTTGAGCTGG
GAAGAGAGGTTGAAGATATCATTAGATGCAGCGCAAGGTTCGGGAAGTCCCATCTTGAAA
TTACATTTAACTAAATCTAACATGCGAAAATCAATCAATGAAGTGTGTGTTTTCTCTGTG
AAAATCAATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
   
Sequence Name 32-K024207-022-365-E12C-8409
GenBank Accession LN500418 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K024207-022-365-E12C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGATTGATTTTCACAGAGAAAACACACAC
TTCATTGATTGATTTTCGCATGTTAGATTTAGTTAAATGTAATTTCAAGATGGGACTTCC
CGAACCTTGCGCTGCATCTAATGATATCTTCAACCTCTCTTCCCAGCTCAAGATAAATGA
CCTTTTACCTTCACAAAATGAAACAAAACCAAAGTTCCTTTCTCAGTACATTATGAACAT
AAGATTAGTGAACAAAAGAAGAATCATTAATTAACCTGCCAAATAGTCTCCTAAGTTCTC
ATTAGCCATATACTCATAGATAAGCACCATGTGGTTTATTTCGTTGCAATATCCAACAAG
AGAAGTCAGGTTCGTGTGATGAACTCTCATGAGAAGGTCAACCTGCGATTCTCAACTAGA
TTAGTTAGGTGAAAAAGTTTGAGATAAATTAAGATTAAGTCCTTGAAAGGTACGCAAAAA
AAACCTCTGCTCGAAACTCTTTGTAGCCTTGAGCTGATTCTTCAGAGAGTACCTTGACAG
CAACTTGTTCT

Last Updated on 06.12.2018 16:11