Sequences from confirmation amplicon


Confirmation sequences for line 366A03 and hit At2g39450 are shown.
Line ID 366A03
Locus At2g39450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16473183
Primers used for PCR HF17 - 8474, show primer details
   
Sequence Name 43-K032417-022-366-A03C-8409
GenBank Accession LN504735 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K032417-022-366-A03C-8409
TTACAATTGAATAATGGAGATGCTTGAGGGCTTTACTGAAATGGATGAACTTGCAGAACG
TGGCTTTGTTCCTGGAATGTCAAAGGTTAGCCTAAATGAGGCTACTACTTGTCACATTTG
TTGAAACCATGATGCATTTGTTCTTGTTGATTGATACTTTTCTGAATTTCTTTGTGTTTT
GTGTACATAGGAAGAGCAGGATAATTTGGCTAAAAGCGAGACATTGGCGATTAGAATATC
AAACATTGCAAACATGCTTCTTTTTGCTGCTAAAGTCTATGCTTCTGTCACTAGTGGCTC
TTTAGCTATCATTGCCTCTACATTGGACTCTCTTCTTGATCTTCCTTTTCTGG
   
Sequence Name 43-K032417-022-366-A03C-HF17
GenBank Accession LN504736 [GenBank]
Sequencing primer gene specific primer, HF17: 5'-GCCAGAAAGAAGATCAAGAAGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K032417-022-366-A03C-HF17
AAGAGCCCTAGTGACAGAAGCATAGACTTTAGCAGCAAAAAGAAGCATGTTTGCAATGTT
TGATATTCTAATCGCCAATGTCTCGCTTTTAGCCAAATTATCCTGCTCTTCCTATGTACA
CAAAACACAAAGAAATTCAGAAAAGTATCAATCAACAAGAACAAATGCATCATGGTTTCA
ACAAATGTGACAAGTAGTAGCCTCATTTAGGCTAACCTTTGACATTCCAGGAACAAAGCC
ACGTTCTGCAAGTTCATCCATTTCAGTAAAGCCCTCAAGCATCTCCATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
CA

Last Updated on 06.12.2018 16:11