Sequences from confirmation amplicon


Confirmation sequences for line 366B01 and hit At1g44780 are shown.
Line ID 366B01
Locus At1g44780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16911874
Primers used for PCR BW58 - 8474, show primer details
   
Sequence Name 01-K106018-0022-366-B01C-BW58
GenBank Accession LN486581 [GenBank]
Sequencing primer gene specific primer, BW58: 5'-ACTGCAACTGATGTGTATGGAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K106018-0022-366-B01C-BW58
TCATCAATCTTGTGGGATGAGGTTTCTACCAAACTTGGTATATCTTTAAGAACTTTTGTA
TTTGTTCATATTCCATGCATTTTATTGTTTTATTATTTTTTCGTGGCAGTGTTCCTCCAA
ACATTTACAAGAAAGCCAAACAGGCTCCTCAAGAGAAACGTGAGGCTATGCTAATAGAAG
AACTTGAGCAGATACTTGCCAAAGAAGGGCTGTCTTCAGATCCTTCAGCGCTAGGTAGTG
TTTCGCTCAGAATTGATTTCTTTTGCGTGGAGAGAAACTTATATCTTCCATCTTTAGAGT
TCAGTAGTTTTTTTCTGAATGATTCTTAATTCAACTTCTGAATATGTTAACCCGAATAAG
CATGCTGATGTTTGTTTTCTATCTGGTAAATGTGTGATGAACATACTATGAACAAACTAT
CTGGCTTTACTCTGACAGAGATCAAAGAAGTAAAAAAGAGGAAGAATATATCAAGAGAGC
TTGAGGGTATTGATACAAACAACATTGTATGGAACTCAAGGAGAAGATCCTCCACAAGTT
TCGCGCCTCCTCCAAAACCGAAAGTAACAGCGGAAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTA
   
Sequence Name 01-K106019-0022-366-B01C-8409
GenBank Accession LN486582 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K106019-0022-366-B01C-8409
TTCGGTTTGGAGGAGGCGCGAAACTTGTGGAGGATCTTCTCCTTGACCTTTATAAATGTT
GTTTGTATCAATACCCTCAAGCTCTCTTGATATATTCTTCCTCTTTTTTACTTCTTTGAT
CTCTGTCAGAGTAAAGCCAGATAGTTTGTTCATAGTATGTTCATCACACATTTACCAGAT
AGAAAACAAACATCAGCATGCTTATTCGGGTTAACATATTCAGAAGTTGAATTAAGAATC
ATTCAGAAAAAAACTACTGAACTCTAAAGATGGAAGATATAAGTTTCTCTCCACGCAAAA
GAAATCAATTCTGAGCGAAACACTACCTAGCGCTGAAGGATCTGAAGACAGCCCTTCTTT
GGCAAGTATCTGCTCAAGTTCTTCTATTAGCATAGCCTCACGTTTCTCTTGAGGAGCCTG
TTTGGCTTTCTTGTAAATGTTTGGAGGAACACTGCCACGAAAAAATAATAAAACAATAAA
ATGCATGGAATATGAACAAATACAGAACTTCTTAAAGATATACCAAGTTTGGTAGAAACC
TCATCCCACAAGATTTGATGACTGATTTGAGATGCTCAACACGCTTTCCATACACATCAG
TTGCAG
Primers used for PCR BW59 - 8474, show primer details
   
Sequence Name 04-K106136-0022-366-B01C-8409
GenBank Accession LN487927 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K106136-0022-366-B01C-8409
TCTGAGATGAAGAGGAAGTAATGAAAAAGCTGAGAGAGGAAGCCAAAGCGAAGAAGTAGA
AGAAGAATCAAACAGCGGTGAGAATCTATATCTCACATCTAAATGACCAACGGGTCAAAT
TTTGAAACTGATGATCCTTGACTTTCTTTGCAGAAGAGGATGATGGTGGAGAAGAAAGCG
ATTGAATTAACGATTATCTCTGACTCAGTGATTGGTGGAGAAGAAAGCGAATGATTTAAC
CATTTTGTTCTTTTAGCCTTTCAAGATTAGGATGAATATTAAGCAGGTAATATGTCAAAC
TTTTAACTATAGTGATAACTAAGTGGGAATCCCAGACTCTGGTTACAACTTGAGGAATCT
TATGCCGAATCACTGAATGTTTTTGATACCCACTCATGNATGTGA
   
Sequence Name 04-K106135-0022-366-B01C-BW59
GenBank Accession LN487926 [GenBank]
Sequencing primer gene specific primer, BW59: 5'-ATCACATCATTGAGTGGGTATCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K106135-0022-366-B01C-BW59
GATCCTCAGTTGTACCAGAGTCTGGGATTCCCACTTAGTTATCACTATAGTTAAAAGTTT
GACATATTACCTGCTTAATATTCATCCTAATCTTGAAAGGCTAAAAGAACAAAATGGTTA
AATCATTCGCTTTCTTCTCCACCAATCACTGAGTCAGAGATAATCGTTAATTCAATCGCT
TTCTTCTCCACCATCATCCTCTTCTGCAAAGAAAGTCAAGGATCATCAGTTTCAAAATTT
GACCCGTTGGTCATTTAGATGTGAGATATAGATTCTCACCGCTGTTTGATTCTTCTTCTA
CTTCTTCGCTTTGGCTTCCTCTCTCAGCTTTTTCATTACTTTCCTCTTCATTCTCAGAAT
CTTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11