Sequences from confirmation amplicon


Confirmation sequences for line 367A04 and hit At1g64110 are shown.
Line ID 367A04
Locus At1g64110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23799442
Primers used for PCR KY01 - 8474, show primer details
   
Sequence Name 39-K026694-022-367-A04C-8409
GenBank Accession LN503224 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K026694-022-367-A04C-8409
TGTAGATTTCCCGGACATGAAGCCATTTACAATTAGTTTCAGCGCCTTTGAAACGAAGTA
GCAGTTGGTCATTCGATGAAAAGCTTCTCGTCCAATCTTTATATAAGGTTATGTGTAAAA
CTATAAAGAGTATAAAGGTAATGTGTTATGTGTAATATCTTGTTTATAATATCTTGCAAA
TGTGAAGTGCAGGTCTTGGCCTATGTCTCCAAGGCGAATCCGATTGTGTTATATCTTCGA
GACGTCGAGAACTTTCTGTTCCGCTCACAGAGAACTTACAACTTGTTCCAGAAGCTTCTC
CAGAAACTCAGTGGACCGGTCCTCATTCTCGGTTCAAGAATTGTGGACTTGTCAAGCGAA
GACGCTCAAGAAATTGATGAGAAGCTCTCTGCTGTTTTCCCTTATAATATCGACATAAGA
CCTCCTGAGGATGAGACTCATCTAGTGAGCTGGAAATCGCAGCTTGAACGCGACATGAAC
ATGATCCAAACTCAGGACAATAGGAACCATATCATGGAAGTTTTGTCGGAGAATGATCTT
ATATGCGATGACCTTGAATCCATCTCTTTTGAGGACACGAAGGTTTTAAGCAATTACATT
GAAGAGATCGTTGTCTCTGCTCTTTCCTATCATCTGATGAACAACAAAGATCCT
   
Sequence Name 40-K026694-022-367-A04C-KY01
GenBank Accession LN503579 [GenBank]
Sequencing primer gene specific primer, KY01: 5'-TTTCCGTTTCTGTACTCAGGATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K026694-022-367-A04C-KY01
GTTGTTCATCAGATGATAGGAAAGAGCAGAGACAACGATCTCTTCAATGTAATTGCTTAA
AACCTTCGTGTCCTCAAAAGAGATGGATTCAAGGTCATCGCATATAAGATCATTCTCCGA
CAAAACTTCCATGATATGGTTCCTATTGTCCTGAGTTTGGATCATGTTCATGTCGCGTTC
AAGCTGCGATTTCCAGCTCACTAGATGAGTCTCATCCTCAGGAGGTCTTATGTCGATATT
ATAAGGGAAAACAGCAGAGAGCTTCTCATCAATTTCTTGAGCGTCTTCGCTTGACAAGTC
CACAATTCTTGAACCGAGAATGAGGACCGGTCCACTGAGTTTCTGGAGAAGCTTCTGGAA
CAAGTTGTAAGTTCTCTGTGAGCGGAACAGAAAGTTCTCGACGTCTCGAAGATATAACAC
AATCGGATTCGCCTTGGAGACATAGGCCAAGACCTGCACTTCACATTTGCAAGATATTAT
AAACAAGATATTACACATAACACATTACCTTTATACTCTTTATAGTTTTACACATAACCT
TATATAAAGATTGGACGAGAAGCTTTTCATCGAATGACCAACTGCTACTTCGTTTCAAAG
GCGCTGAAACTAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCATATATGGAGAAAAAGAAAGAG

Last Updated on 06.12.2018 16:11