Sequences from confirmation amplicon


Confirmation sequences for line 367A08 and hit At3g09740 are shown.
Line ID 367A08
Locus At3g09740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2991116
Primers used for PCR 38my - 8474, show primer details
   
Sequence Name 02-K114418-0022-367-A08C-8409
GenBank Accession LN487133 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K114418-0022-367-A08C-8409
ATTTCATTGAATATATCCTGGACTCTCAGCCACATAGGTCTGTTCTTGATACCTAATATA
AAAGCATTGTCTTTTGTTACAGCTGAGATCTAGCCGGAACTTCTGTATCGATATTGTTTT
GTTGTGTATTGTTCTGGGTATCGCTGCATACTTATACAAGTAAGTTTCTCTCATACGTTT
CGATTATAGTTATTCACTCTGAAAAAACTTGTTTAACGAGACAAACATTTGTTCTCTGTG
CAGTGTACTGAAGTAATGAGATGAACCCTACGAAAGGACCCATTAGTACTTATCACCCGA
GTCAATATCCNGTGTGTGCTGNTGTCTAA
   
Sequence Name 26-K114419-0022-367-A08C-38my
GenBank Accession LN498036 [GenBank]
Sequencing primer gene specific primer, 38my: 5'-GTAAGACACAAGCACACACTGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K114419-0022-367-A08C-38my
GTTCATCTCATTACTTCAGTACACTGCACAGAGAACAAATGTTTGTCTCGTTAAACAAGT
TTTTTCAGAGTGAATAACTATAATCGAAACGTATGAGAGAAACTTACTTGTATAAGTATG
CAGCGATACCCAGAACAATACACAACAAAACAATATCGATACAGAAGTTCCGGCTAGATC
TCAGCTGTAAACAAAAGACAATGCTTTTATATTAGGTATCAAGAACAGACCTATGTGGCT
GAGAGTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11