Sequences from confirmation amplicon


Confirmation sequences for line 367C06 and hit At4g22290 are shown.
Line ID 367C06
Locus At4g22290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11784712
Primers used for PCR 55dh - 8474, show primer details
   
Sequence Name 29-K109642-0022-367-C06C-55dh
GenBank Accession LN499329 [GenBank]
Sequencing primer gene specific primer, 55dh: 5'-CTAACCTCTGCATGTCTAGATCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K109642-0022-367-C06C-55dh
GGGTTTGCGGATTTCCCACAAACCTTTGTATTCATACAACTCCGTGGATACATAAACGCA
TCTTGGTGTTCTTTGGAACGAAGATTCCAAAGGAATGCTTCCACATGTCACAACCTACAA
AAACATAACAAAAGTTTTAACATTTCCAGCAAATAAAAGACACACCAGTGACCACACTAC
CATATCTACATCAACATTGTTAATTCAGACAATATAAATGGTCTGTCCAACGCATTAAGC
TAGCTGTTGACGACTGTAATCCAATCGACAATACAAAAATACCCGTGACTCCAATTTGTC
GAATTATAAATGGTCTAATTTCACTAAAGACTCTTATAAAATCTATACTAAACACAAGCA
GCAAACTTTAGTTTGGTGAGATAATATAAAATGTCAAAGACAGACAAAAGCAAAAATCTA
CATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 13-K109633-0022-367-C06C-8409
GenBank Accession LN492081 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K109633-0022-367-C06C-8409
CATTTACATTGAATATATCATGTAGATTTTTGCTTTTGTCTGTCTTTGACATTTTATATT
ATCTCACCAAACTAAAGTTTGCTGCTTGTGTTTAGTATAGATTTTATAAGAGTCTTTAGT
GAAATTAGACCATTTATAATTCGACAAATTGGAGTCACGGGTATTTTTGTATTGTCGATT
GGATTACAGTCGTCAACAGCTAGCTTAATGCGTTGGACAGACCATTTATATTGTCTGAAT
TAACAATGTTGATGTAGATATGGTAGTGTGGTCACTGGTGTGTCTTTTATTTGCTGGAAA
TGTTAAAACTTTTGTTATGTTTTTGTAGGTTGTGACATGTGGAAGCATTCCTTTGGAATC
TTCGTTCCAAAGAACACCAAGATGCGTTTATGTATCCACGGAGTTGTATGAATACAAAGG
TTTTGGTGGGAAATCCGCAAACCCAAAACATCGATGCTTTTCTTGGGGATCTAGACATGC
AGAGGTTAG

Last Updated on 06.12.2018 16:11