Sequences from confirmation amplicon


Confirmation sequences for line 368C02 and hit At1g49430 are shown.
Line ID 368C02
Locus At1g49430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18292126
Primers used for PCR SR05 - 8474, show primer details
   
Sequence Name 01-K027360-022-368-C02C-8409
GenBank Accession LN486432 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K027360-022-368-C02C-8409
CTTTCATTGATATATACGTACCGATCAATATATATACAACATTTTATCTTAGGCTTGTCT
TCATATTATATATATTCATGAGTTATGAAAACCTAATCCGAGGTTGAATGTCGATGTAAC
AGGCGTAAACGCAGTTGAATTCATCATCAACCACGCCGAGGTTTCGCTAGTATTTGTTCA
AGAGAAGACAGTTTCATCGGTAAGTCAATTTTTTTAATTGAAATCAGTCATACCATAACT
TTCATTTTAGGTTTCTTGTCCAACAAGAAAGTATTTTTGTTTTGCTTTTGTAATTAACTC
TTGAAAGTTTTTCTGCAGATCTTATCGTGCCAAAAGGGATGTTCTTCGAATTTGAAGAGT
AAGTTACATGTCTTTAAAAATCGATAATATTTTGTATAAATATGATCTTAAAATCTAAGA
TAGTTCTTTTGCTT
   
Sequence Name 02-K027360-022-368-C02C-SR05
GenBank Accession LN486891 [GenBank]
Sequencing primer gene specific primer, SR05: 5'-AAACCGTAAGTCAAAGCAAAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K027360-022-368-C02C-SR05
TATTTATACAAAATATTATCGATTTTTAAGACATGTAACTTACTCTTCAAATTCGAAGAA
CATCCCTTTTGGCACGATAAGATCTGCAGAAAAACTTTCAAGAGTTAATTACAAAAGCAA
AACAAAAATACTTTCTTGTTGGACAAGAAACCTAAAATGAAAGTTATGGTATGACTGATT
TCAATTAAAAAAATTGACTTACCGATGAAACTGTCTTCTCTTGAACAAATACTAGCGAAA
CCTCGGCGTGGTTGATGATGAATTCAACTGCGTTTACGCCTGTTACATCGACATTCAACC
TCGGATTAGGTTTTCATAACTCATGAATATATATAATATGAAGACAAGCCTAAGATAAAA
TGTTGTATATATATTGATCGGTACGTATATATCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
Primers used for PCR FN57 - 8474, show primer details
   
Sequence Name 85-K118629-0022-368-C02C-FN57
GenBank Accession LN514592 [GenBank]
Sequencing primer gene specific primer, FN57: 5'-GCGTTGATCCGGTACATTATTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K118629-0022-368-C02C-FN57
TTTATTTGAAGACATTGGCCAAAAAAAAGGTTCTTATTTTTCTTACTTTTTTTGTGGAGT
AGGGACACTGTTGTGGTATTTACGGAGCTAATTGTCCAGAATGGATTATTGCAATGGAGG
TAAAAAAAAATTGACCTATATCTAAAATTTAGGGTTCAATTTTCCTTTTTATTATAGAGT
TTTTTTTGTTTTGAAAGAAATATAGATGATTGAGATTGAGAAGGGCCTTTTGTTATAGAG
TTTCTCTAAGTTGTTATATTGCAGGCCTGCATGAGCCAAGGGATCACCTACGTGCCTCTA
TACGTGCCTGTATATATCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 66-K118628-0022-368-C02C-8409
GenBank Accession LN511710 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K118628-0022-368-C02C-8409
TTGAATAATCCTGGATATATACGGCACGTATAGAGGCACGTAGGTGATCCCTTGGCTCAT
GCAGGCCTGCAATATAACAACTTAGAGAAACTCTATAACAAAAGGCCCTTCTCAATCTCA
ATCATCTATATTTCTTTCAAAACAAAAAAAACTCTATAATAAAAAGGAAAATTGAACCCT
AAATTTTAGATATAGGTCAATTTTTTTTTACCTCCATTGCAATAATCCATTCTGGACAAT
TAGCTCCGTAAATACCACAACAGTGTCCCTACTCCACAAAAAAAGTAAGAAAAATAAGAA
CCTTTTTTTTTGGCCAATGTCTTTAAAATAAATAATATACTTGATTGATTGGATCATGTC
ATAACATAGTAATAATGTACCGGATCAAC

Last Updated on 06.12.2018 16:11