Sequences from confirmation amplicon


Confirmation sequences for line 368C08 and hit At1g71410 are shown.
Line ID 368C08
Locus At1g71410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26913343
Primers used for PCR 15dr - 8474, show primer details
   
Sequence Name 75-K110174-0022-368-C08C-15dr
GenBank Accession LN513258 [GenBank]
Sequencing primer gene specific primer, 15dr: 5'-GCCAACTTACCAACAGAAAATGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K110174-0022-368-C08C-15dr
CAGAGAAAGTATACATGAAAATAGCGTAGCCGCCGTAAAATAATATTATGTGTGACAAAT
GAAGCAATGGTGAGAAAACATAACAAAATTTGTACAATTTGAGAAAGACATAAAACAACC
TGAAGCTGTAGTAAAGTTTATATAGTGGAAGAAGCTAATCCCAGAAGATGTTTACATAAC
ATCTCCATTTTTCTGAACAGTTTTCTCTGCATCACAAAGCACGGAGCGCAATCTCCATGT
GTATATGCTTCTCTTAATCACACGACAAAACACCAACACCACACCAAGAGGTTCAACACC
AAGAACTGTGGTCATAATAGATCCAATAGAGATGGTTGTGAACCACTGGGCTTTGAGGTA
GATGTGCCAGTACCACCTCTACCTCTACCCCTTCCTCTTCCCATTGCTATTGAAGGTGGT
GGTGCGAGTTTCATTGCGGACGGTTCGGTTTTGCTTGAACCGAATATGGAACTGATGTCT
GCTGGCTTCTGGTTGTTGTATGAACCACTGGTGAAAGATGGCATTCCTTGGCTCTGTTTT
GGTACACCGAAAGAGTTGAGTGGATCTTGATTATTTGCAAGATATATTAAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 65-K110173-0022-368-C08C-8409
GenBank Accession LN511423 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K110173-0022-368-C08C-8409
GAGCCATTTACAATTTAATATATCTTGCAAATAATCAAGATCCACTCAACTCTTTCGGTG
TACCAAAACAGAGCCAAGGAATGCCATCTTTCACCAGTGGTTCATACAACAACCAGAAGC
CAGCAGACATCAGTTCCATATTCGGTTCAAGCAAAACCGAACCGTCCGCAATGAAACTCG
CACCACCACCTTCAATAGCAATGGGAAGAGGAAGGGGTAGAGGTAGAGGTGGTACTGGCA
CATCTACCTCAAAGCCCAGTGGTTCACAACCATCTCTATTGGATCTATTATGACCACAGT
TCTTGGTGTTGAACCTCTTGGTGTGGTGTTGGTGTTTTGTCGTGTGATTAAGAGAAGCAT
ATACACATGGAGATTGCGCTCCGTGCTTTGTGATGCAGAGAAAACTGTTCAGAAAAATGG
AGATGTTATGTAAACATCTTCTGGGATTAGCTTCTTCCACTATATAAACTTTACTACAGC
TTCAGGTTGTTTTATGTCTTTCTCAAATTGTACAAATTTTGTTATGTTTTCTCACCATTG
CTTCATTTGTCACACATAATATTATTTTACGGCGGCTACGCTATTTTCATGTATACTTTT
TCTTCTTTTGGCAGTTCCTGGATCATTTTCTGTTG

Last Updated on 06.12.2018 16:11