Sequences from confirmation amplicon


Confirmation sequences for line 368D02 and hit At2g29900 are shown.
Line ID 368D02
Locus At2g29900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12750182
Primers used for PCR 20et - 8474, show primer details
   
Sequence Name 21-K111225-0022-368-D02C-20et
GenBank Accession LN495716 [GenBank]
Sequencing primer gene specific primer, 20et: 5'-TCTCTCCAAACTCTTCTCTGAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K111225-0022-368-D02C-20et
AGCCGGGATGTCTTCGTCTCTAGATATAGCCATTTCAACAAGAAGACGTAATGGACCAAC
AGGTAAAAGAACAGCAGCAATGTCATAAAGTGCCAAAGCTACGAGAAGAACCCAAGTAGT
CCATTCAGGTAACAACGTGAAGAAGTAAGCAACTAAAACACCAATCCAAACCAAGTAACC
TTGAGTGATCAAAATCGAAAACTTCGACATGAACACAGCGAAAACACCAACGACGGAGAA
ATTGAAGAGAAGTATCAAAAACGTGATAGAATCAATCGGGAATCTAAATCGATCTATCAA
TAAAACTAAGATTTCTCCACCTAAGTTCCCATGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 05-K111224-0022-368-D02C-8409
GenBank Accession LN488414 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K111224-0022-368-D02C-8409
ATGGGAACTTAGGTGGAGAAATCTTAGTTTTATTGATAGATCGATTTAGATTCCCGATTG
ATTCTATCACGTTTTTGATACTTCTCTTCAATTTCTCCGTCGTTGGTGTTTTCGCTGTGT
TCATGTCGAAGTTTTCGATTTTGATCACTCAAGGTTACTTGGTTTGGATTGGTGTTTTAG
TTGCTTACTTCTTCACGTTGTTACCTGAATGGACTACTTGGGTTCTTCTCGTAGCTTTGG
CACTTTATGACATTGCTGCTGTTCTTTTACCTGTTGGTCCATTACGTCTTCTTGTTGAAA
TGGCTATATCTAGAGACGAAGACATCCCGGCTTTGGTTTACGAAGCACGGCCTGTGATCC
GAAATGATTCCCGTTCGGTTCAGAGAAGAGTTTGGAGAGA

Last Updated on 06.12.2018 16:11