Sequences from confirmation amplicon


Confirmation sequences for line 368G12 and hit At4g37320 are shown.
Line ID 368G12
Locus At4g37320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17560122
Primers used for PCR GE08 - 8474, show primer details
   
Sequence Name 16-K033704-022-368-G12C-8409
GenBank Accession LN493337 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K033704-022-368-G12C-8409
ATTTACATTGAATATGTTAACAAACGTAAACGAGGTTTCTGACACGATACAAACGTAAAA
TACGTGGAGTAGTAGTGGAACCGCACGATACAAACGTAATGTTTCTGACACAATGTTTTG
GAGATAATGGAGATTTACAATATCTGATTCTTCAACTAACCGGTCTGAACCGATTTTATC
ATCGATCTCCGCTCTCGCTTTCTCAAGTATCTCTGGATGGTTCAGCAAATTTGACATTGC
CCATTCCAACGTCACTGAAGATGTATCTGTCCCCGCAAGAACCAAAGCCTGTGTATATAT
ATATCTCTAATAAGTAAACCCACTCCACATATAAGGAGGAT
   
Sequence Name 16-K033704-022-368-G12C-GE08
GenBank Accession LN493338 [GenBank]
Sequencing primer gene specific primer, GE08: 5'-TATCCTCCTTATATGTGGAGTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K033704-022-368-G12C-GE08
TACACGGCTTTGGTTCTTGCGGGGACAGATACATCTTCAGTGACGTTGGAATGGGCAATG
TCAAATTTGCTGAACCATCCAGAGATACTTGAGAAAGCGAGAGCGGAGATCGATGATAAA
ATCGGTTCAGACCGGTTAGTTGAAGAATCAGATATTGTAAATCTCCATTATCTCCAAAAC
ATTGTGTCAGAAACATTACGTTTGTATCCTGCGGTTCCACTACTACTCCACGTATTTTAC
GTTTGTATCCTGTCAGAAACCTCGTTTACGTTTGTTAACATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAA
ATGG

Last Updated on 06.12.2018 16:11