Sequences from confirmation amplicon


Confirmation sequences for line 369C03 and hit At4g11330 are shown.
Line ID 369C03
Locus At4g11330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6893636
Primers used for PCR NE37 - 8474, show primer details
   
Sequence Name 62-K102433-0022-369-C03C-8409
GenBank Accession LN510723 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K102433-0022-369-C03C-8409
ATTTACATTGAATTGTAGATTTCCGGGTATGATGTCCGGGTGATGAATCCTTGATCATAC
TCTTTGTTTTGCAGTTGAGGAAGCACTGTGTTATCCTTACTTGTCAGCTCTTCACGACCT
TAACGACGAGCCTGTTTGTTCCAACCATTTCAGTTTCCATTTTGAGGATCCGTCTTCCAC
TGAAGAAGAGATAAAGGAGCTTGTGTGGCTCGAATCTGTGAAGTTCAATCCTCTGCCGAG
CATTTAAAACATTATTTAAGAAGGGGAAAGCAAGTTTTATACTCTGACATTGAGCAAAAC
AAATTAGTAGTGTTTAAGCTCTGTTTCTCCTTCATCTCATGTTGATCGTTGTTTAAG
   
Sequence Name 62-K102434-0022-369-C03C-NE37
GenBank Accession LN510724 [GenBank]
Sequencing primer gene specific primer, NE37: 5'-GCTTAAACAACGATCAACATGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K102434-0022-369-C03C-NE37
TACAATTTGTTTTGCTCAATGTCAGAGTATAAAACTTGCTTTCCCCTTCTTAAATAATGT
TTTAAATGCTCGGCAGAGGATTGAACTTCACAGATTCGAGCCACACAAGCTCCTTTATCT
CTTCTTCAGTGGAAGACGGATCCTCAAAATGGAAACTGAAATGGTTGGAACAAACAGGCT
CGTCGTTAAGGTCGTGAAGAGCTGACAAGTAAGGATAACACAGTGCTTCCTCAACTGCAA
AACAAAGAGTATGATCAAGGATTCATCACCCGGACATCATACCCGTGAAATCTACAATTT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGT
CTACATTCAC

Last Updated on 06.12.2018 16:11