Sequences from confirmation amplicon


Confirmation sequences for line 369F08 and hit At1g05270 are shown.
Line ID 369F08
Locus At1g05270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1532814
Primers used for PCR 14os - 8474, show primer details
   
Sequence Name 59-K114954-0022-369-F08C-14os
GenBank Accession LN510068 [GenBank]
Sequencing primer gene specific primer, 14os: 5'-TCTGTTCTCAAACCTCAAACATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K114954-0022-369-F08C-14os
TTCCATTTGGTTTTCTTCTTTGTATATTGTGAAGATCTATCATTGTGAGTGATTTTGGAA
AAAATGCATATCATTGTGTGATGATTAGTTTTGTTAGTGTCTCTTTCTATCTTTAGAATG
ATTAGTGTTGCCAAAATTTCTTCACTAAATCACAAAGCAGAGGCTGCCTATATCTTAGAG
GAAATTTCTATGATGCTTGTACATCACTTGCTCTATATGCTATGGCTTTGGTGATGAGAT
TTCACAGATCTAAGTACTTATACACGGTTCTAGCCCTTTTGCTTATTCTGAAATGGTAAT
ATATTTGCTTTTCTAGATTCCAACCATGTCGGACATGATAGAAAGTTGGAAGCAGAAACA
GAACACATTCGGAATACTTTATGGATGGTTTCTTGCAAAGGCAGGTTTTCCTTAATCAAG
AAACTTTTTGATGGAAGCTTTAAGTCTCTGAACCAAAAATTTACATCCTTTCCTTCTGTT
AGATCGCCAGTCATCTTGAGGTTTTTCCTGGTGCTGAGTTTCGTGTGGCGTATGAAGAGG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 25-K114953-0022-369-F08C-8409
GenBank Accession LN497571 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K114953-0022-369-F08C-8409
AACCTCAGATGACTGGCGATCTACAGAAGGAAAGGATGTAAATTTTTGGTTCAGAGACTT
AAAGCTTCCATCAAAAAGTTTCTTGATTAAGGAAAACCTGCCTTTGCAAGAAACCATCCA
TAAAGTATTCCGAATGTGTTCTGTTTCTGCTTCCAACTTTCTATCATGTCCGACATGGTT
GGAATCTAGAAAAGCAAATATATTACCATTTCAGAATAAGCAAAAGGGCTAGAACCGTGT
ATAAGTACTTAGATCTGTGAAATCTCATCACCAAAGCCATAGCATATAGAGCAAGTGATG
TACAAGCATCATAGAAATTTCCTCTAAGATATAGGCAGCCTCTGCTTTGTGATTTAGTGA
AGAAATTTTGGCAACACTAATCATTCTAAAGATAGAAAGAGACACTAACAAAACTAATCA
TCACACAATGATATGCATTTTTTCCAAAATCACTCACAATGATAGATCTTCACAATATAC
AAAGAAGAAAACCAAATTGGAAAGAAGAAGCAGAATCACAATTGTGAAAGGCAAACCTTC
AATGTTTGAGGTTGAAAAACAG

Last Updated on 06.12.2018 16:11