Sequences from confirmation amplicon


Confirmation sequences for line 370B11 and hit F1N21 are shown.
Line ID 370B11
Locus F1N21 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25173450
Primers used for PCR AB36 - 8474, show primer details
   
Sequence Name 08-K103785-0022-370-B11C-AB36
GenBank Accession LN489700 [GenBank]
Sequencing primer gene specific primer, AB36: 5'-AATATTTTGCGATCTAATTCCACG-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K103785-0022-370-B11C-AB36
TTGGATGATCCCACCGATTTAGATCTGTCTCGATTTTTTAATGTCCATGATCATGAATAT
GAGTAAAAGCGGTAAGAATCTAGAGGTGCATGTATAATATATGTATGGATACTGAATATT
TTAGTTTTGATTATTCGAATCTGACATGAAAACATATATTATTAGTTTTTATACAATGAC
AAAAGCATAATGTATACAATCACGTTTTGAAGTTCATGCTTGCCTGAGGAGACTTCGAAG
TCCATGTAGATCTTGAAAATTTGTTCGCAAAGATAGTTAATTAAAGTGCTATCAAACTAG
CTAACAATTTAATGTTTCATGTAAGTATGAAAATATTGAGGAAGAGCATTACTTCATAAA
GCACAAGATGTTTGATGGGACAATTGAAGCTTTACAAACTTTAAGATAACATTGCATATA
TCTCCACCAAATCATTATCAAAAGGCCAACTTGAATTACTTTGCTCGAAGTTCGGGTTGA
CTGACATAATCAGATTTAAGGTAGTGTTAAATTTAAACTTGATAATGATTGATGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACNTGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
   
Sequence Name 24-K103786-0022-370-B11C-8409
GenBank Accession LN496960 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K103786-0022-370-B11C-8409
ATTTACATTGAATATATCCATCAATCATTATCAAGTTTAAATTTAACACTACCTTAAATC
TGATTATGTCAGTCAACCCGAACTTCGAGCAAAGTAATTCAAGTTGGCCTTTTGATAATG
ATTTGGTGGAGATATATGCAATGTTATCTTAAAGTTTGTAAAGCTTCAATTGTCCCATCA
AACATCTTGTGCTTTATGAAGTAATGCTCTTCCTCAATATTTTCATACTTACATGAAACA
TTAAATTGTTAGCTAGTTTGATAGCACTTTAATTAACTATCTTTGCGAACAAATTTTCAA
GATCTACATGGACTTCGAAGTCTCCTCAGGCAAGCATGAACTTCAAAACGTGATTGTATA
CATTATGCTTTTGTCATTGTATAAAAACTAATAATATATGTTTTCATGTCAGATTCGAAT
AATCAAAACTAAAATATTCAGTATCCATACATATATAATACATGCACCTCTNGATTCTTA
CCGCTTTTACTCATATTCATGATCATGGACATTTAAAAAATCGAGACAGATCTNAAATCG
GTGTGGATCATCCAAATAACTTGTCCACTCCTACTTCACATCGTGGAATTAGATCGCANA
ATA

Last Updated on 06.12.2018 16:11