Sequences from confirmation amplicon


Confirmation sequences for line 371F10 and hit At3g44900 are shown.
Line ID 371F10
Locus At3g44900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16390477
Primers used for PCR 14lu - 8474, show primer details
   
Sequence Name 03-K114086-0022-371-F10C-14lu
GenBank Accession LN487575 [GenBank]
Sequencing primer gene specific primer, 14lu: 5'-GTTATGCGTATCAAAGAGGGGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K114086-0022-371-F10C-14lu
TATCTACACATTTTGATACACTCAGAAACTGAGCTTAGTATTCATTTTGATTGCAGACTA
TTCGGCCGGTGACCTTTACGGTTTTATCTTTGTACATCTTGTTGAACTCTGCAGTCATAC
CTCCGCTCTTAAAACGCATTTATGATCCTTCGAGGATGTACGCAGGATACGAGAAGCGGA
ACATGCTTCACATGAAACCAAACTCCGAGCTCAGGATTCTATCGTGTATCTACAAAACAG
ACGATATCCGTCCGATGATCAATCTCCTTGAAGCTACTTGTCCCTCAAGAGAAAACCCTG
TAGCAATGAGCAATGAGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 27-K114085-0022-371-F10C-8409
GenBank Accession LN498484 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K114085-0022-371-F10C-8409
GCATTTACATTGAATATACTCATTGCTCATTGCTACAGGGTTTTCTCTTGCGGTACAAGT
AGCTTCAAGGAGATTGATCATCGGACGGATATCGTCTGTTTTGTAGATACACGATAGAAT
CCTGAGCTCGGAGTTTGGTTTCATGTGAAGCATGTTCCGCTTCTCGTATCCTGCGTACAT
CCTCGAAGGATCATAAATGCGTTTTAAGAGCGGAGGTATGACTGCAGAGTTCAACAAGAT
GTACAAAGATAAAACCGTAAAGGTCACCGGCCGAATAGTCTGCAATCAAAATGAATACTA
AGCTCAGTTTCTGAGTGTATCAAAATGTGTTAGATACATTTTGAAGGAAATATGTCAAAT
ACATTTACTTACCCCTCTTTGATACGCATAA

Last Updated on 06.12.2018 16:11