Sequences from confirmation amplicon


Confirmation sequences for line 371H02 and hit At1g57590 are shown.
Line ID 371H02
Locus At1g57590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21328257
Primers used for PCR 12kq - 8474, show primer details
   
Sequence Name 60-K113756-0022-371-H02C-12kq
GenBank Accession LN510313 [GenBank]
Sequencing primer gene specific primer, 12kq: 5'-ACCTTTTACCAAATTCACCATACG-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K113756-0022-371-H02C-12kq
ATTTCAAACATTTGTTAGTTTGGTTATTCAGAATAAGAACATAAGTCGGAAAAAAAAATC
AAGAACATCATTATTAGCTTATGTTTCACCTTGCAAGAATTGGATCTGAGATGCACTACA
CTTGGCGTAGTTAAGTCTACAATCATTCCAACTTCCACTTGGATCTGCAGATTTTGGAGC
TAAACTCTCTTGAATCTGCACCAAAAAAAAACTAACTAAACACAAAATCTTGTTAATAAA
TCTGATGATCAACAAGAGAATTACTTACCTGCCAAGAATCATATGCAGCATTAAGAATAA
AAAGTGGAGTCTTGACTTGGTTGATTAGGTTTTGTGGGAAAAAACACTGCAATATTGAAA
CCATGGAGTACATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCACGATTATTAA
   
Sequence Name 22-K113755-0022-371-H02C-8409
GenBank Accession LN496197 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K113755-0022-371-H02C-8409
ACTCCTGGTTTCATATTGCAGTGTTTTTTCCCACAAAACCTAACCATCCAAGTCAAGACT
CCACTTTTTATTCTTAATGCTGCATATGATTCTTGGCAGGTAAGTAATTCTCTTGTTGAT
CATCAGATTTATTAACAAGATTTTGTGTTTAGTTAGTTTTTTTTTGGTGCAGATTCAAGA
GAGTTTAGCTCCAAAATCTGCAGATCCAAGTGGAAGTTGGAATGATTGTAGACTTAACTA
CGCCAAGTGTAGTGCATCTCAGATCCAATTCTTGCAAGGTGAAACATAAGCTAATAATGA
TGTTCTTGATTTTTTTTTCCGACTTATGTTCTTGATTCTGAATAACCAAACTAACAAATG
TTTTTGAAATGTGTGTTACAGGTTTTAGGACGCGTATGGTGAATTGGTTAAAAGGT

Last Updated on 06.12.2018 16:11