Sequences from confirmation amplicon


Confirmation sequences for line 371H11 and hit At4g19120 are shown.
Line ID 371H11
Locus At4g19120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10462716
Primers used for PCR JK47 - 8474, show primer details
   
Sequence Name 19-K100938-0022-371-H11C-8409
GenBank Accession LN494683 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K100938-0022-371-H11C-8409
TTTACATTGAATATACTAATGTTTTGATTTCGTTTTGCTTGTTCAGAAATGGAAGAAGTA
TGGTACTCACAGGCTTACTTTCATGGAGCGACATTGTCCTCCTGTGTTTGATAGAAAGCA
GTGTCTGGTTCCTCCTCCTGATGGCTATAAGCCACCGATAAGATGGCCAAAGAGCAAAGA
CGAATGTTGGTACAGGTATATGTTTGTCCCTTTTCTCAACCTTATGATTGCATCTNCTAA
TTACTTGTATCTCTAAATCTCTCCTGTCT
   
Sequence Name 19-K100944-0022-371-H11C-JK47
GenBank Accession LN494684 [GenBank]
Sequencing primer gene specific primer, JK47: 5'-AACTTTTCACCCTCTTTCCTTAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K100944-0022-371-H11C-JK47
TCCATCAATGGCACATTCCTGAAATCAAATTACCAACTTACTTCAAATAGCTGAAGACAG
GAGAGATTTAGAGATACAAGTAATTAAGAGATGCAATCATAAGGTTGAGAAAAGGGACAA
ACATATACCTGTACCAACATTCGTCTTTGCTCTTTGGCCATCTTATCGGTGGCTTATAGC
CATCAGGAGGAGGAACCAGACACTGCTTTCTATCAAACACAGGAGGACAATGTCGCTCCA
TGAAAGTAAGCCTGTGAGTACCATACTTCTTCCATTTCTGAACAAGCAAAACGAAATCAA
AACATTAGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAG

Last Updated on 06.12.2018 16:11