Sequences from confirmation amplicon


Confirmation sequences for line 372B03 and hit At4g23390 are shown.
Line ID 372B03
Locus At4g23390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12219694
Primers used for PCR GE01 - 8474, show primer details
   
Sequence Name 22-K033705-022-372-B03C-GE01
GenBank Accession LN496015 [GenBank]
Sequencing primer gene specific primer, GE01: 5'-TAGAATGGTTCTTGAGCAAAGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K033705-022-372-B03C-GE01
AGTCAATATATAACCATGTTCAGTCTGTCGAAATCAAAAGCAAGAAAAAATTGGTGTGGT
CTGAAAATAAATGTAACTTGATGATAGTTGTAATATTGTTACAATAGTCAACAATTTTTG
TAATGTTTTATGAAAATTTAAGAAAAGAAAGGCTAAATTTACTTGGAAACTTTTGACTGC
AGGTTTATTGAGATAGGTAAGTAGCTTCTTCATCTCTTCTTTCTCCTCTTTCGAAGGTAT
TGTTTTGTGACTCTCTCCAACTAAAATGAGAGCTATTGTTGATAAAGTTAGCAAGATAAT
TAGTTTATTGGAATACCTCATGATGAATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC
   
Sequence Name 22-K033705-022-372-B03C-oriL
GenBank Accession LN496016 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K033705-022-372-B03C-oriL
TTACAATTGAATATATTCATCATGAGGTATTCCAATAAACTAATTATCTTGCTAACTTTA
TCAACAATAGCTCTCATTTTAGTTGGAGAGAGTCACAAAACAATACCTTCGAAAGAGGAG
AAAGAAGAGATGAAGAAGCTACTTACCTATCTCAATAAACCTGCAGTCAAAAGTTTCCAA
GTAAATTTAGCCTTTCTTTTCTTAAATTTTCATAAAACATTACAAAAATTGTTGACTATT
GTAACAATATTACAACTATCATCAAGTTACATTTATTTTCAGACCACACCAATTTTTTCT
TGCTTTTGATTTCGACAGACTGAACATGGTTATATATTTGACTGCATCGACATTCAAAAA
CAGCTAGCTTTTGATCATCCTTTGCTCAAGAACCATTCT
Primers used for PCR GE02 - 8474, show primer details
   
Sequence Name 23-K033705-022-372-B03C-8409
GenBank Accession LN496460 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K033705-022-372-B03C-8409
TTACATTGAATATATACTCATGATGATAATTAGAACAACTATATTTATTGTGTCACATAA
ATCTGAGATCATTTATAACCACCAAAGAACCTATACACAGTAAATGACAAATGTATCTCC
CTCTATCTCTATTGCCCATATGTAGATGCTAAAGTAAGATTTCTCTTTTTTTTAATGTAC
TTTTTTTTGTATAAAGTATATTCCATAAGAAAAAGGAAAAGCTTGTTTATGGATCAATTG
ACCCCAAAAAAAGTTTTTAGATCAAAGCCCAATATAAAAAAAAAACACAGTAGTGACACA
AAGGAACTTAAATAAACCATGAATTGATCTATAAACAGTAAAGATCGATAAGGCAAAA
   
Sequence Name 23-K033705-022-372-B03C-GE02
GenBank Accession LN496461 [GenBank]
Sequencing primer gene specific primer, GE02: 5'-TTCGCCTTATCGATCTCTACTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K033705-022-372-B03C-GE02
GTTATTTAGTCCTTTGTGTCACTACTGTGTTTTTTTTTTATATTGGGCTTTGATCTAAAA
ACTTTTTTTGGGGTCAATTGATCCATAAACAAGCTTTTCCTTTTTCTTATGGAATATACT
TTATACAAAAAAAAGTACATTAAAAAAAAGAGAAATCTTACTTTAGCATCTACATATGGG
CAATAGAGATAGAGGGAGATACATTTGTCATTTACTGTGTATAGGTTCTTTGGTGGTTAT
AAATGATCTCAAATTTATGTGACACAATAAATATAGTTGTTCTAATTATCATCATGAGTA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATTCACG

Last Updated on 06.12.2018 16:11