Sequences from confirmation amplicon


Confirmation sequences for line 372C05 and hit At2g37010 are shown.
Line ID 372C05
Locus At2g37010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15542900
Primers used for PCR 12du - 8474, show primer details
   
Sequence Name 57-K110361-0022-372-C05C-12du
GenBank Accession LN509449 [GenBank]
Sequencing primer gene specific primer, 12du: 5'-TCTGATTTATGTAAACGAAGCGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K110361-0022-372-C05C-12du
CGCTTGCCAGCTTATAAACAACAGGATTTTGTGAGCCGTAAGTTGTTAGCTTTCTAGATT
GAGTAAGTTAATGAAAAATCTTATTAGTGGATGATTTAAAGAGTTTTTACATTGCAGATA
TAACTACCAAATTCCTCCGGGAAAGTTGAATCATACATGTGGTTCTGCAGATAGTTGGGT
TGATGCTGAGAGTAGTGGTGATATGTTTTGTTCACCAGGTTCATATTGCCCAACAACGAT
TCGAAAAGTCACTTGTAGTAGTGGGTATTGATTCTTTTATCTCACAACTCTTGTTACTTT
TTGAAAATTTGTTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
C
   
Sequence Name 41-K110360-0022-372-C05C-8409
GenBank Accession LN504113 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K110360-0022-372-C05C-8409
CAATTTTCAAAAGTAACAAGAGTTGTGAGATAAAAGAATCAATACCCACTACTACAAGTG
ACTTTTCGAATCGTTGTTGGGCAATATGAACCTGGTGAACAAAACATATCACCACTACTC
TCAGCATCAACCCAACTATCTGCAGAACCACATGTATGATTCAACTTTCCCGGAGGAATT
TGGTAGTTATATCTGCAATGTAAAAACTCTTTAAATCATCCACTAATAAGATTTTTCATT
AACTTACTCAATCTAGAAAGCTAACAACTTACGGCTCACAAAATCCTGTTGTTTTATTAA
GCTTGGCAAGCGGGCAATATGCGCCTAACGGACACGCTTCGTTTACATAAATCA

Last Updated on 06.12.2018 16:11