Sequences from confirmation amplicon


Confirmation sequences for line 372F04 and hit At4g28840 are shown.
Line ID 372F04
Locus At4g28840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14240968
Primers used for PCR PC88 - 8474, show primer details
   
Sequence Name 16-K103845-0022-372-F04C-PC88
GenBank Accession LN493404 [GenBank]
Sequencing primer gene specific primer, PC88: 5'-CATTTCGATCAACAAGAAGAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K103845-0022-372-F04C-PC88
TTCTGCATTCGATTAGACATTACGTCTCAAACACAGACAAAAAAAAATACAGTTAGCTAT
CACGATCAAAAAGAAAATTGCATCCAAATCATCCTTAATGTCACGGTAGAAAGATTCTAA
ACCAGAGTTTTTTTGATGAAATTCTAAAATTTATAAAAAACAATTACTACTAGACTATTT
TTACATGGATCTATAGAGATATGTATATGTGAGTGAAATTTGTACCTGATAAGTTGAGGG
ATGATAAGAAGGGTAGGTATTGAAGCTGTTGCAGTTATACTCGCCGTGTAATCTAATCTT
CTCTAACTGCGCGACGCCAAGACCTCTCTAAGGCTGCTTAGGCTTGTCAGAACCAATTAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATTTCCG
   
Sequence Name 32-K103846-0022-372-F04C-8409
GenBank Accession LN500555 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K103846-0022-372-F04C-8409
GCCATTTACAATTGAATATATAATTGGTTCTGACAAGCCTAAGCAGCCTCAGAGAGGTCT
CGGCGTCGCGCAGTTAGAGAAGATTAGATTACACGGCGAGTATAACTGCAACAGCTTCAA
TACCTACCCTTCTTATCATCCCTCAACTTATCAGGTACAAATTTCACTCACATATACATA
TCTCTATAGATCCATGTAAAAATAGTCTAGTAGTAATTGTTTTTTATAAATTTTAGAATT
TCATCAAAAAAACTCTGGTTTAGAATCTTTCTACCGTGACATTAAGGATGATTTGGATGC
AATTTTCTTTTTGATCGTGATAGCTAACTGTATTTTTTTTTGTCTGTGTTTTGAGACGTA
ATGTCTAAATCGAATTGCAAAATGATTTAAATTCAGCATGCGTTTGTTTCTTCTTGTTGA
TCGAAAT
Primers used for PCR 07sk - 8474, show primer details
   
Sequence Name 41-K116239-0022-372-F04C-07sk
GenBank Accession LN504214 [GenBank]
Sequencing primer gene specific primer, 07sk: 5'-CTGCAATTCGATTTAGACATTACG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K116239-0022-372-F04C-07sk
CACGATCAAAAAGAAAATTGCATCCAAATCATCCTTAATGTCACGGTTAGAAAGATTCTA
AACCAGAGTTTTTTTTGATGAAATTCTAAAATTTATAAAAAACAATTACTACTAGACTAT
TTTTACATGGATCTATAGAGATATGTATATGTGAGTGAAATTTGTACCTGATAAGTTGAG
GGATGATAAGAAGGGTAGGTATTGAAGCTGTTGCAGTTATACTCGCCGTGTAATCTAATC
TTCTCTAACTGCGCGACGCCAAGACCTCTCTGAGGCTGCTTAGGCTTGTCAGAACCAATT
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGC
   
Sequence Name 17-K116238-0022-372-F04C-8409
GenBank Accession LN494008 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K116238-0022-372-F04C-8409
TTACATTGAAATATATAATTGGTTCTGACAAGCCTAAGCAGCCTCAGAGAGGTCTCGGCG
TCGCGCAGTTAGAGAAGATTAGATTACACGGCGAGTATAACTGCAACAGCTTCAATACCT
ACCCTTCTTATCATCCCTCAACTTATCAGGTACAAATTTCACTCACATATACATATCTCT
ATAGATCCATGTAAAAATAGTCTAGTAGTAATTGTTTTTTATAAATTTTAGAATTTCATC
AAAAAAACTCTGGTTTAGAATCTTTCTACCGTGACATTAAGGATGATTTGGATGCAATTT
TCTTTTTGATCGTGATAGCTAACTGTATTTTTTTTTGTCTGTGTTTTGAGACGTAATGTC
TAATCGNAATTGCAG

Last Updated on 06.12.2018 16:11