Sequences from confirmation amplicon


Confirmation sequences for line 373B10 and hit At3g10280 are shown.
Line ID 373B10
Locus At3g10280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3181566
Primers used for PCR AY35 - 8474, show primer details
   
Sequence Name 05-K020608-022-373-B10C-8409
GenBank Accession LN488164 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K020608-022-373-B10C-8409
TAGATTTCCCGGACATGAAGCCATTTATGAACCGAGTCACCCCACGGGTTACCCACCAAT
GACTCGTTAGCCGGAATCGTTCTCAATGCTCTCCACACAATACTATTACATTTAAAACCC
GACCCAAACGCCAACTGCCAAACCCTATCTCCTCGTTTGACCCGACATTTAGCCTCCACG
TAAGCCAGCTCGTACCACAGCGACGAGCTTGAAGTGTTTCCAAAGCGGTGCAAAGTCATC
CGAGACGGCTCAAGATCAAACTCACTAAGTCCAAGACCTTTTTCAACCGCGTCAAGTAAC
GCTCTACCACCAGCGTGAATACAGAAATGCTTGAAACATAGTTTAAAATCCGGAACATAT
GGACTAACCTTTAACCGGAAAAGCTTACTCTTTACCAAAAAGAGGATAAACCGGAGTTTT
TCAGATAAAGGCAAAACAAGCGGACCAAGTGCTGTTAGATTCGTCTTTAACGTGTCACCA
GCAACAACCGTTAGTTCTTTAGAGAGTGCGACTCCAACGATACCTTTACTATCTTCTTTT
TGTTCAGCACAAGTGTAGTGTTTATCGCTAGAGCCTTTGTGAGTCCTTACAATATGAGTC
AATTCGTACTTAGACCGGACTCGGTCTTGACTCCGGTTTGAGAGCATAACCGCAGCGCCA
CCTACTCGGAATAGACAGTTAGGGACTAACATTGACCGGTCGTTGCCTCGATACATGCTT
AGAGTCATGTTCTCCGTACTAACAATAACTGC
   
Sequence Name 06-K020608-022-373-B10C-AY35
GenBank Accession LN488611 [GenBank]
Sequencing primer gene specific primer, AY35: 5'-GACCTCGCCACAAATCTACTAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K020608-022-373-B10C-AY35
CCTAATACCTACGCAGTTATTGTTAGTACGGAGAACATGACTCTAAGCATGTATCGAGGC
AACGACCGGTCAATGTTAGTCCCTAACTGTCTATTCCGAGTAGGTGGCGCTGCGGTTATG
CTCTCAAACCGGAGTCAAGACCGAGTCCGGTCTAAGTACGAATTGACTCATATTGTAAGG
ACTCACAAAGGCTCTAGCGATAAACACTACACTTGTGCTGAACAAAAAGAAGATAGTAAA
GGTATCGTTGGAGTCGCACTCTCTAAAGAACTAACGGTTGTTGCTGGTGACACGTTAAAG
ACGAATCTAACAGCACTTGGTCCGCTTGTTTTGCCTTTATCTGAAAAACTCCGGTTTATC
CTCTTTTTGGTAAAGAGTAAGCTTTTCCGGTTAAAGGTTAGTCCATATGTTCCGGATTTT
AAACTATGTTTCAAGCATTTCTGTATTCACGCTGGTGGTAGAGCGTTACTTGACGCGGTT
GAAAAAGGTCTTGGACTTAGTGAGTTTGATCTTGAGCCGTCTCGGATGACTTTGCACCGC
TTTGGAAACACTTCAAGCTCGTCGCTGTGGTACGAGCTGGCTTACGTGGAGGCTAAATGT
CGGGTCAAACGAGGAGATAGGGTTTGGCAGTTGGCGTTTGGGTCGGGTTTTAAATGTAAT
AGTATTGTGTGGAGAGCATTGA

Last Updated on 06.12.2018 16:11