Sequences from confirmation amplicon


Confirmation sequences for line 374C08 and hit At5g67610 are shown.
Line ID 374C08
Locus At5g67610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26962971
Primers used for PCR 24ni - 8474, show primer details
   
Sequence Name 06-K118075-0022-374-C08C-24ni
GenBank Accession LN489000 [GenBank]
Sequencing primer gene specific primer, 24ni: 5'-ATATCCATGGACTTGGAAGGTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K118075-0022-374-C08C-24ni
TTAACGCGTCATCTCTGGGAAAACAATAACATTGACGTTTGCTTTCACCGGTGAGTCCTT
CGTTTATCTTTTGCTGTTGGACTTAAATTCTTGATATTCCTGTAAGTTCGTTGGTACATA
ATAGATATCACTAAATGTTGTATGTTTGAAGGAATTTGTCGCGTGGAATTGGAATGTGCC
CTCATAGTCGATGGGAGAAAGCCTCTAAAGGTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTC
   
Sequence Name 01-K118074-0022-374-C08C-8409
GenBank Accession LN486769 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K118074-0022-374-C08C-8409
CTTTCTCCCATCGACTATGAGGGCACATTCCAATTCCACGCGACAAATTCCTTCAAACAT
ACAACATTTAGTGATATCTATTATGTACCAACGAACTTACAGGAATATCAAGAATTTAAG
TCCAACAGCAAAAGATAAACGAAGGACTCACCGGTGAAAGCAAACGTCAATGTTATTTGT
TTTCCCAGAGATTGACGCGTTAACTATCAACTTGAGCGAATGTGCATACTTGTCTAGATG
TTTAAACCTTCCAAGTCCCATGGATAT

Last Updated on 06.12.2018 16:11